[Histonet] alexa fluors

[Gayle Callis]

There is only one supplier of Alexa fluorophores - Molecular Probes part of 
Invitrogen, and you can go to their website to find an Alexa which will 
excite at the wavelength you want to use.  Contact their technical services 
for recommendations on this, they are very helpful.  Molecular Probes 
Handbook is on line, explaining anything you want to know about Alexa 
fluorophores including their array of Alexa related products.  You will not 
be disappointed.

At 03:25 AM 1/4/2006, you wrote:
>Hi all,
>
>I need to do some triple localisation studies.  Previously, I have used 
>FITC, Cy3 and AMCA.  I have never really liked AMCA that much, fades 
>quickly and the colour is pretty dire.  I'm wondering about using an Alexa 
>fluor which excites either in the blue or near red spectrum.  Can you 
>recommend one or the other and a reliable supplier ?!
>
>Many thanks
>
>Patrick
>----------------------
>Patrick Howorth,
>Dept of Physiology,
>University of Bristol,
>Bristol,
>BS8 1TD.
>+44 (0)117 33 17112
>patrick.howorth <@t> bristol.ac.uk
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)