[Histonet] Uneven Staining

[Kemlo Rogerson]

We had a linear stainer once and uneven staining was a problem; look at
dewaxing and complete immersion in haematoxylin and eosin. We ultimately
moved to another platform.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

"One of the benefits of getting older is finding wisdom but  so many get of
us get older and never find it; sadly that is a waste of a life." 

 

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-----Original Message-----
From: Mary Parker [mailto:mparker <@t> epl-inc.com] 
Sent: Monday, February 27, 2006 5:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Uneven Staining

Can anyone tell me if they have had uneven staining (H&E) on a linear
stainer?

 

This one is relatively new to our lab and we are having a very trying
time especially with liver, brain and heart sections.

 

We have done all our usual problem solving and ruled out fixation,
improper de-paraffinization, and microtoming artifacts.

 

The pattern is variable, inconsistent and random. When we use the same
staining set up and do the slides by hand we get better results.

 

Any suggestions would be appreciated.

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