[Histonet] Clearing Colon Fat to Reveal Lymph Nodes

[Rene J Buesa]

Sandy:
  I used the following solution to harden the lymph nodes and make them more evident to tact (more "revealed"):
  a- 100% ethanol -------- 2.0 litres
  b- distilled water -------- 0.68 litres
  c- 40% formalin --------- 0.32 litres
  d- acetic acid ------------ 0.2 litres 
  (Total volume = 3.2 litres)
  It worked quite well!
  Hope this will help you!
René J.
sandosis <@t> uia.net wrote:
  Hello,

Could you folks please let me know what methods and reagents you have 
worked with that do a decent job of overnight clearing the fat from 
colonectomy specimens to further reveal lymph nodes? 

Many Thanks,
Sandy

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