[Histonet] H&E staining

[Monfils, Paul]

The responses suggest that some people (most, probably) are using an
alcoholic eosin solution and others are using aqueous eosin.  We use
eosin/phloxine in 80% ethanol, followed by 20 dips in one 95% ethanol and 20
dips in each of three absolute alcohols, then into xylene.  "Dips" is not a
very precise measurement, but our rate is about 2 dips per second, so the
slides are in each dish about 10 seconds.  This provides a total of 30
seconds immersion in absolute alcohol with constant agitation, which is
plenty to dehydrate a slice of tissue 5 microns thick.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> eileen dusek
> Sent: 	Monday, February 27, 2006 8:30 AM
> To: 	histonet
> Subject: 	[Histonet] H&E staining
> 
> Hi Everyone,
>   This is a basic question.
>   How long are tech leaving slides in 95% and 100% ETOH after eosin. I
> feel we are in too long but would like to get other ideas.
>   Our slides have been very washed out, little to no contrast.
>    
>   I appreciate your help
>    
>   Eileen C. Dusek
>   Edward Hospital
> 
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