[Histonet] Re: Wright-Giemsa stain on tissue

[Laurie Reilly]

Dear Dina and Histonetters,
Giemsa stain on tissue sections was used some time ago looking for 
Erhlichia canis  in dog tissues.
The method was to make a very weak Giemsa solution and stain overnight.
We used five drops of Giemsa in a Coplin jar of tap water ( ours is pH 5.5)
Differentiate next day in very weak acetic acid.

               Regards,  Laurie.

At 01:07 PM 22/02/2006 -0600, Johnson, Teri wrote:
>Dina,
>
>In my days in clinical labs, we did a May-Grunwald Giemsa stain on our
>bone marrow biopsies and particle preps. I'm with Dr. Richmond in that
>there the staining results from the Zenker's fixed material was second
>to none.  However, we were able to do a reasonably good job of it,
>provided the pathologists never had to pull archival slides to compare.
>That being said, I am happy to never go back to using it, what a pain!
>
>You might try using the M-G Giemsa technique and see how it works for
>you.
>
>Teri Johnson, HT(ASCP)QIHC
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, MO 64133
>
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Mr.Laurie Reilly                                              Ph 07 4781 4468
School of Veterinary & Biomedical Science      Fax  07 4779  1526
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.