[Histonet] processing

[Kellar, Eric C]

Dorothy,

I infiltrate my GI biopsies with a low melting point paraffin (Paraplast Xtra) at 58*C on my VIP processors. GI biopsies easily become friable at paraffin temperatures of 62*C and over. Ask her to check the temps of her paraffins between the two processors. Also, GI biopsies seem to be more challanging to cut after fresh dehydrating alcohol solutions are added to the processors. They respond well to graduated alcohols from 70%, 80%, 95%, 100% to the clearant. I do not use the Ventana processor, but I do know it does require some fine tuning to program the dehydrating alcohols to match those on the VIP.

Eric C. Kellar
Histology/Immunohistochemistry
Quest Diagnostics Inc. Miami


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Webb,
Dorothy L
Sent: Wednesday, February 22, 2006 12:12 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing


I am submitting this for a colleague at another institution who has a
concern over GI biopsies.  They have 2 processors they use, one VIP and
one Renaissance, both programmed with the same times and the same
reagents at each station.  They use recycled formalin.  Their
pathologists are complaining that the Gi biopsies that come off of the
Renaissance processor are not of good quality, chattered, drier, etc.
and the problems are not seen on the slides of the biopsies from the
other (VIP) processor. They had a vendor out to check out the processor
in question, inasmuch as they could be getting carry over of reagents,
etc. and all checked out ok.  Any suggestions as to the cause of their
problem?  Thanks ahead of time from a lead tech who is pulling out her
hair!!!!  I told her to "hold on", as my fellow histonetters would come
up with something!!
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