[Histonet] RE: AMACR (P504S) (Lori Harris)

lharris <@t> samhealth.org lharris <@t> samhealth.org
Mon Feb 20 16:03:32 CST 2006


Richard,

I found your response very interesting but could you explain to me what CMS & MUE stand for? Thanks!

Lori A. Harris, HT (ASCP)
Histology Section Leader
GSRMC - Pathology
3600 NW Samaritan Drive
Corvallis, OR 97330
1-541-768-6078
lharris <@t> samhealth.org



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Sent: Sunday, February 19, 2006 10:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 27, Issue 29


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Today's Topics:

   1. P-cadherin (Richard Cartun)
   2. Helicobacter Pylori (Adesupod <@t> aol.com)
   3. Re: AMACR (P504S) (Richard Cartun)


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Message: 1
Date: Sun, 19 Feb 2006 10:09:22 -0500
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] P-cadherin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s3f843d5.007 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

Is anyone doing IHC for P-cadherin on formalin-fixed, paraffin-embedded
tissue?  If so, where do you get your antibody?  Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax


                                        




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Message: 2
Date: Sun, 19 Feb 2006 11:33:12 EST
From: Adesupod <@t> aol.com
Subject: [Histonet] Helicobacter Pylori
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <27.3d00fc2.3129f7c8 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

                                                                              
                                                                  
   Hi,
      Pls I want you histonetters to tell me the best silver technique for 
the demonstration of the Helicobacter Pylori.
  You guys are the best.

       Adesupo. 


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Message: 3
Date: Sun, 19 Feb 2006 12:05:22 -0500
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] AMACR (P504S)
To: <histonet <@t> lists.utsouthwestern.edu>,<Michele_Marggi <@t> ssmhc.com>
Message-ID: <s3f85f21.075 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

I agree with Bob Richmond's comments.  Our high molecular weight
cytokeratin (clone 34BetaE12) works beautifully for demonstrating an
absence of basal cells around malignant glands.  We use P504S (from
DAKO) on rare cases.  Their antibody (clone 13H4) is labeled "IVD".  I
know that many labs are running multiple IP stains (HMW-CK, p63, P504S,
and others) on prostate biopsies.  To me, this is completely unnecessary
for the majority of complicated cases where a diagnosis cannot be
established on H&E stains, and is one reason why CMS is proposing MUEs
to limit the number of IP stains that can be performed on a patient's
specimen.

Richard


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> <Michele_Marggi <@t> ssmhc.com> 02/14/06 11:24AM >>>

I am researching AMACR (P504s) and need some help.  Who out there in
histo-land is using it?  Is this a Research Use Only antibody?  Is a
disclaimer in the report required?  Any input would be
appreciated......

Thanks,

Michele Marggi
Surgical Pathology Supervisor
St. Marys Hospital Medical Center
707 S Mills Street
Madison WI  53715
Telephone: 608.258.6930
Fax: 608.258.6268


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