[Histonet] Chick embryo freezing

[Margaryan, Naira]

Thanks Teri,

Problem is: I have to fix/cut or cut/fix the chick embryos with GFP
labeled cells into; and then use these fluorescent labeled cells
sections for the laser capture microdissection and microarray analysis. 
This technique is a new for us. 
I did send my question to Arcturus Company and do not have any answer
yet.

Any help will be gratefully accepted,
Naira


Naira V. Margaryan, D.V.M., Ph.D.
Research Associate III
Children's Memorial Research Center
2300 Children's Plaza, Box 222
Chicago, IL 60614-3394
Tel: 773-755-6570/ext-8
Fax: 773-755-6594
nmargaryan <@t> childrensmemorial.org
 
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Naira,

We will formalin fix and cryopreserve chick embryos before freezing in
OCT with good results. What sorts of issues are you having? What age
sample do you have?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133