[Histonet] Aluminium in paraffin embedded sections

[Kemlo Rogerson]

The aurine technic of PC Irwin was recommended by Lillie; page 534 of his
book Hitopathologic Technic and Practical Histochemistry.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 
 
 

-----Original Message-----
From: Lina Vieira [mailto:livieira <@t> ualg.pt] 
Sent: Thursday, February 16, 2006 3:45 PM
To: Histonet
Subject: [Histonet] Aluminium in paraffin embedded sections 


We want identify Aluminium in paraffin embedded sections of fish spleen and
kidney for light microscopy. 
I found diferent techniques and want your advise about it.
I indicate the technique that seems easy to me:
(from Pearse, 1972)
Solution A . 0.2% solochrome azurine (aqueous)
Solution B. 0.2% solochrome azurine in normal sodium hydroxide
Method
1. Take two sections to water
2. Stain one section in each solutions - 20 minutes
3. Wash in water
4. Counterstain in 1% neutral red - 5 minutes
5. Wash in tap water
6. Dehydrate through gradde alcohols to xylene
7 Mount in DPX

Resultes
section stain with solution A - Aluminium and beryllium - blues
section stain with solution B - Beryllium - blue-black 

Thanks in advance for any information you can provide,

Lina Vieira
University of Algarve
Portugal

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