[Histonet] whole brain in paraffin

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Thu Feb 16 08:18:02 CST 2006


WOW a Tetrander!!!

Thought those things had been consigned to the torture chamber.....

Used that thing 25 years ago with mega brain slices, hardest bit was keeping
the block cool but as long as the processing was Ok (used to take weeks to
process the brain) it was not too bad; needed skill in manipulating the
sections and then floating onto dirty great big coated slides. If you have
any probs then contact me. 

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 
 
 

-----Original Message-----
From: Nancy Lemke [mailto:nsnwl <@t> neuro.hfh.edu] 
Sent: Thursday, February 16, 2006 1:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole brain in paraffin

Good Morning Histonet,
I would like some information about the preparation and sectioning of 1-2cm
slabs of human brain.  The cutting will be done on an old Reichert Tetrander
(sliding) microtome with a giant steel blade.  If anyone who has experience
with this sort of sectioning would email me I would love to ask some
questions.
Thank you in advance,
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit

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