[Histonet] heat resistant staining nets
Runyan, Caroline (NIH/NIMH) [F]
runyanc <@t> mail.nih.gov
Wed Feb 15 13:29:30 CST 2006
Hello,
I normally perform DAB staining on free-floating brain sections in
staining nets from brain research labs. However, I need to add an
antigen retrieval step at the beginning, 15 min in citrate buffer at
90C. I've just tested the nets that I have, and they will not stand up
to the heat. Does anyone know of a heat-resistant staining net? Thank
you.
Caroline
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