[Histonet] Re: Suggestions for sectioning mouse colon

[Johnson, Teri]

David, it sounds as though you are doing everything right! You might try
adding 1 drop of Photoflo to your flotation bath (or some alcohol) to
break the surface tension just a little. It could be your paraffin will
only relax to a certain extent, too and nothing will help short of
trying a different paraffin.  I don't know if a few ripples/nips are
worth that headache.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133