[Histonet] PLGA frozen section problem

[Ashwin Nair]

Hi,

I am trying to section Poly (lactide-co-glycolide){PLGA} scaffolds and
subsequently stain them. I am unable to cut wax sections. I tried embedding
the scaffold in OCT by placing the scaffold in a mould with OCT for 1 hour
under vacuum 20psi. Then the mould was placed in the freezer. 5 & 10
micron sections were cut.
However, the sections that I managed to cut using the cryostat tend to be
folded and also disintegrate while staining.
Has anyone encountered this.
I don't have this problem with PLLA scaffolds though as wax sections work
fine.

Please help.

Ashwin