[Histonet] Alcohol Based EDTA Decalcifiers

[Kemlo Rogerson]

I don't know if this makes sense but alcohol is non-polar liquid isn't it?
Will EDTA work in a non-polar environment? I think the alcohol will suppress
ionisation and hence not work; calcium ions are also insoluble in alcohol.

10% solution of EDTA in 0.1M Tris buffer PH 6.95 may be the solution (get
it?).

Best wishes 


Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 
 
 

-----Original Message-----
From: Etheridge, Sandra AL:EX [mailto:Sandra.Etheridge <@t> gov.bc.ca] 
Sent: Tuesday, February 14, 2006 10:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Alcohol Based EDTA Decalcifiers

Hello, everyone,

Is anyone aware of a commercial or homemade alcohol based EDTA
decalcifying solution?  Our fish pathologist is currently fixing his
tissues in Davidson's and would prefer to go into an alcohol decal
solution instead of an aqueous one (acid hematin
pigment forms around the red cells - not nice for photos).

Any information is, as always, greatly appreciated.

Thanks!

Sandra Etheridge

BC Ministry of Agriculture & Lands
Animal Health Center, Histology
Abbotsford, BC
Canada

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