[Histonet] HELP, Plastic sectioning

[Molinari, Betsy]

I use the Leica RM 2155 automated microtome and a D profile tungsten
carbide knife . My angle is approx. 4 degrees, but I have to adjust that
accordingly. I cut at 3um.
Be sure everything is tight, tight tight.
Good luck.

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sunhwa
Lee
Sent: Monday, February 13, 2006 12:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HELP, Plastic sectioning

I am trying to do plastic sectioning with Fish brain and the section
is shattered a lot.  I've been changing thickness 1-5um, new knife,
and embedding materials (JB-4 and Technovit 7100), but not thing
works. My slicing conditions are as follows.  Any suggestions, please.
 In advance, thank you so much.

1. Knife : High profile stainless steel knife
2. Microtome: normal rotating Microtome (Leica 820-II)
3. Thickness: 1-5um
4. Cutting angle: 2-3degree
5. Embedding material: GMA (Technovit 7100)
6. Sample: fish brain

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