[Histonet] RE: Mouse tissue staining with monoclonal mouse Abs.

Mon Feb 13 03:14:20 CST 2006

<BODY>Dear Guillermo, Perhaps you should
 try a biotin-free detection system instead of t In my hands endogenous biotin cannot be effectively kidney tissue. Molecular Probes/Invitrogen has a Zenon kit in vitro labeling your mouse primary antibody with either enzymes fluorochromes via a labeled&nbsp;Fab anti-mouse reagent. Then c ontinue either with fluorescence microscopy or anti-FITC for enzymatic
 de van der Loos, PhD Dept. of Pathology Acade Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterd am The Netherlands &nbsp;
 Date: Fri, 10 Feb 2006 15:11:55 +0100
 (CET) From: &lt;gpbnas <@t> yahoo.es&gt; Subject: [ Mouse tissue staining with monoclonal mouse Abs. Hel Vector Mouse on Mouse kit. To: Histonet &lt;histone t <@t> lists.utsouthwestern.edu Dear
 all, &nbs with mouse frozen sections o implying an important mouse IgG kidney). For this reason we bought avoid detection of deposited IgG by seconda maintaining sensitivity for mouse primary antibody. increasing concentration of blocking reagent as well as decreasing concentration of biotynilated anti-mouse IgG, there still is a
 "back glomeruli togeth I would really appre helpful. &nbsp;&n advance, Gui Ph.D. Laboratorio de Reumatología Hospital 12 de Octubre Avda de Córdoba s/n Madrid
 28041 Spain </BODY>