[Histonet] background in lymphoid tissue

[Patsy Ruegg]

Laura,
It can be very difficult to quench endogenous peroxidase in some
tissue/cells, neutrophils in spleen and inflamation are notorious for being
difficult to quench.  If you can you might try using an alk.phos detection
system to see if it is the endogenous peroxidase giving you these problems.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johns, Laura
[CNTUS]
Sent: Friday, February 10, 2006 11:04 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] background in lymphoid tissue

Hi All,

I am having a problem with background staining in lymphoid tissue.  I have
tried increasing the time of my peroxide block, and adding in an
avidin/biotin blocking step, but still there is some slight staining in my
negative controls (no primary or secondary antibodies).  I have noticed this
in spleen and in other organs where there is an area of inflammation, and
with several different antibody combinations.  Has anyone experienced this
problem?  Any suggestions for getting rid of it?

Thanks!
Laura

Laura M. Johns, Ph.D.
Centocor, Inc.
145 King of Prussia Road (Mail Stop # R-4-2) Radnor, PA 19087
Phone:  610-240-8284 ....  Fax:  610-240-8150....  Email:
ljohns53 <@t> cntus.jnj.com

> Confidentiality Notice:  This message is intended for the 
> individual(s) or entity to which it is addressed and may contain 
> information that is privileged, confidential and exempt from disclosure
under applicable law.
> If the reader of this message is not the intended recipient, he/she is 
> hereby notified that any dissemination, distribution or copying of 
> this communication is strictly prohibited.  If you have received this 
> communication in error, please notify the sender by telephone and 
> delete the e-mail from your system immediately.  Thank you.
> 
> 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet