[Histonet] brain tissue (human) to stay on the slides?

[Cheryl]

I know of no scientific explanation to this method but from experience, you usually have to scrape the tissue off with a blade if you want it to come off afterward:
   
  Double Baking:
   
  Oven dry your slides for the usual time.  Pull them out and let them cool COMPLETELY.  Then put them back in the same oven for the same amount of time, and cool completely again.  It seems to 'settle' the tissue against the slides by heating and cooling twice.  This works for most soft tissues. Decal and nail sections didn't work as completely. 
   
  This will effect IHC but works beautifully with no stain changes for H&E and most specials.
   
  Also--always use treated slides, and watch the speed of your rinse waters/aggitation.  
   
  Hope this helps!!
   
  Cheryl Kerry
  Full Staff Inc.
  Staffing the AP Lab by helping one tech at a time. 

Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
  Usually is a problem with processing, or too thick a section, or not "dried" (heated) enough after section or a combination.
René J.

Barb Nuernberger wrote:
Does anyone have any suggestions for getting brain tissue (human) to
stay on the slides? 
Fixation?

Barb

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





---------------------------------
Brings words and photos together (easily) with
PhotoMail - it's free and works with Yahoo! Mail.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet