[Histonet] B-Gal endogenous expression

[John A. Kiernan]

Fairly recently:
Dimri et 10 al (1995) Proc. Natl Acad. Sci. USA
92:9363-9367.
More recently:
Debacq-Chainlaux et 11 al (2005) J. Cell Sci. 118:
743-758.

The lysosomal enzyme has a pH optimum about 4.
Senescent cells contain a different enzyme active
about pH 6. (The enzyme of the lac-Z reporter gene
works at pH 7+ so it's not confused with the
lysosomal enzyme activity.) In the major earlier
papers on galactosidase histochemistry
(1960s-'70s) the illustrations mainly showed
intestine, with strong staining in epithelial
cells; also renal tubules if I remember rightly.
Hope this helps. 
                                    John.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
"Andrea T. Hooper" wrote:
> 
> Does anyone have references or anecdotal experience as to where
> mammalian B-Gal is endogenously expressed if anywhere in mouse or
> human?
> 
> Thanks,
> Andrea
> --