[Histonet] plastic embedded bone sections

[Nancy W. Troiano]

We routinely cut MMA embedded mouse and rat tibias using a 160 mm D-profile 
tungsten carbide microtome blade.  A good source of excellent 
blades/resharpening service is Dorn/Hart microedge (Ken Hart), tel 
#630-832-3843.  Ken is also a great source of information as to the proper 
blade angles, blades, etc for your application.  I am not familiar with the 
microm microtomes but we use a Leica 2165 motorized microtome (you 
definitely need a motorized unit) and set our blade angle at approximately 
2.5 to 3 (number setiing on side of knife holder).  You may have to change 
your angles as the blade is resharpened.