[Histonet] epithelial differentiation-paraffin embedded tissue

[Annette Ekblond]

Dear Histonet subscribers

We find it difficult to identify the following antigens in paraffin embedded epithelium:

Laminin 1
Laminin 5
Transglutaminase 1
Collagen VII

It works for us in cryopreserved specimens - but not in paraffin.......

Does anyone have any suggestions - or perhaps even successfull protocols to solve this problem?


Sincerely

Annette
Coloplast Research
Denmark