[Histonet] Automated ABDPAS

Thu Feb 9 08:14:56 CST 2006

Does anyone automate their alcian blue diastase periodic acid schiffs? Do  you do the whole or part of the stain on the stainer? Are your solutions fresh or reused?  Any advice would be appreciated as we are looking to automate ABDPAS and giemsa for gastric biopsies.

Sarah
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Today's Topics:

   1. re: Technovit 7100 resin removal (Carl Hobbs)
   2. RE: Celestine blue B (Sharon Allen)
   3. argyrophil and argentaffin (Till, Renee)
   4. re: argyrophil and argentaffin (bhewlett <@t> cogeco.ca)
   5. re: argyrophil and argentaffin (bhewlett <@t> cogeco.ca)
   6. Alcohol Disposal (Samuel.Jones2 <@t> med.va.gov)
   7. RE: Celestine blue B (Rittman, Barry R)
   8. Fwd: Re: [Histonet] Alcohol Disposal (Atoska S. Gentry)
   9. backup cryostat (Steven Coakley)
  10. infrared vs uv in sequencing (Malcolm McCallum)
  11. Alcohol Disposal--a few choices (Cheryl)
  12. request feedback on benchtop tissue processors (meint002)
  13. Re: request feedback on benchtop tissue processors (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 8 Feb 2006 14:07:58 -0000
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re: Technovit 7100 resin removal
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201c62cb9$10d89880$112b5c9f <@t> Carlos>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

In all my years, I have never been able to remove the resin ( of ANY 
GMA-based resin) and yet still retain the section fixed to a slide, sigh!
I bought the same Technovit resin for immunohistochemical applications yet have never found it to be a viable alternative to pwax sections.
    Methyl methac is the only resin  that can be removed, IMHO, still 
retaining the section on the slide.
Carl 

------------------------------

Message: 2
Date: Wed, 8 Feb 2006 08:34:14 -0600
From: Sharon Allen <SAllen <@t> exchange.hsc.mb.ca>
Subject: RE: [Histonet] Celestine blue B
To: 'Julien Lambrey de Souza' <julien_lambreydesouza <@t> uqar.qc.ca>,
	"Histonet (E-mail)" <histonet <@t> pathology.swmed.edu>
Message-ID:
	<36FE435D6D2F1D489174B22362A961B66B8313 <@t> hscxntmx0006.hsc.mb.ca>
Content-Type: text/plain; charset="us-ascii"

Hi,
I started using Celestine Blue in our method for Gomori's Trichrome & it
made a great improvement. I'm not sure why!
Put the slides in Celestine Blue for 2 min. before the Mayer's Haematoxylin.
Recipe is:
Ferric ammonium sulfate...........5.0 gm
Dissolve in distilled water.......100 ml.
Celestine blue....................0.5 gm
Boil for 3 min. to dissolve, filter, when cooled add-
Glycerin..........................14 ml
Stable for at least 6 months
(Carleton's Histological Techniques 4th edition  P.133)
Hope this helps.
Sharon
sallen <@t> hsc.mb.ca 
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------------------------------

Message: 3
Date: Wed, 8 Feb 2006 08:43:26 -0600
From: "Till, Renee" <TillRenee <@t> uams.edu>
Subject: [Histonet] argyrophil and argentaffin
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID:
	<11F927674DEBDC43B960809A7403C5D232866C <@t> MAILPED.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii

I had heard that if you are doing a Grimelius for argyrophil cells, that
an argentaffin stain should also be performed to be able to
differentiate between the two cells. Is this true for any argyrophil
stain? 

Renee' Till, HT

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR 

72202

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Message: 4
Date: Wed, 08 Feb 2006 10:13:41 -0500
From: bhewlett <@t> cogeco.ca 
Subject: re: [Histonet] argyrophil and argentaffin
To: "Till,Renee" <TillRenee <@t> uams.edu>,
	histonet <@t> lists.utsouthwestern.edu 
Message-ID: <43ea0aa5.322.b62.22262 <@t> cogeco.ca>

> Renee,

All argentaffin cells are also argyrophil by definition.
Therefore, to distinguish the true argyrophil population, one should perform both stains and subtract the argentaffin
population from the argyrophil.

Bryan

> I had heard that if you are doing a Grimelius for argyrophil cells, that
> an argentaffin stain should also be performed to be able to
> differentiate between the two cells. Is this true for any argyrophil
> stain?
>
>
>
> Renee' Till, HT
>
>
>
> Arkansas Childrens Nutrition Center
>
> 1120 Marshall
>
> Little Rock, AR
>
> 72202
>
>
>
>
>
>
> ===============================================================================================
> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended
> recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or
> distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and
> destroy all copies of the original message.
> ===============================================================================================_______________________
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> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 5
Date: Wed, 08 Feb 2006 10:15:24 -0500
From: bhewlett <@t> cogeco.ca 
Subject: re: [Histonet] argyrophil and argentaffin
To: "Till,Renee" <TillRenee <@t> uams.edu>,
	histonet <@t> lists.utsouthwestern.edu 
Message-ID: <43ea0b0c.40.65c1.3974 <@t> cogeco.ca>

> Renee,

All argentaffin cells are also argyrophil by definition.
Therefore, to distinguish the true argyrophil population, one should perform both stains and subtract the argentaffin
population from the argyrophil.

Bryan

> I had heard that if you are doing a Grimelius for argyrophil cells, that
> an argentaffin stain should also be performed to be able to
> differentiate between the two cells. Is this true for any argyrophil
> stain?
>
>
>
> Renee' Till, HT
>
>
>
> Arkansas Childrens Nutrition Center
>
> 1120 Marshall
>
> Little Rock, AR
>
> 72202
>
>
>
>
>
>
> ===============================================================================================
> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended
> recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or
> distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and
> destroy all copies of the original message.
> ===============================================================================================_______________________
> ________________________ Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 6
Date: Wed, 8 Feb 2006 09:08:12 -0600 
From: Samuel.Jones2 <@t> med.va.gov 
Subject: [Histonet] Alcohol Disposal
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID:
	<476BAB689BEA3E4DBE6F8D5A90CAC2CF056FC7DA <@t> vhantxexc1.v17.med.va.gov>
Content-Type: text/plain; charset="us-ascii"

Hello All:

I've been asked by my peers to see how labs are collecting and disposing of
their waste alcohol. Thoughts please. Thanks

Samuel E. Jones, MS, HT(ASCP)HTL, QIHC

Supervisor, Anatomic Pathology

VA North Texas Health Care System

4500 South Lancaster Road, 113

Dallas, Texas 75216

Phone: 214-857-0659

e-mail: samuel.jones2 <@t> med.va.gov <mailto:samuel.jones2 <@t> med.va.gov> 

------------------------------

Message: 7
Date: Wed, 8 Feb 2006 09:42:40 -0600
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] Celestine blue B
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EA1FDD2A141B7448B4B1AFFFCAC08DE405DBBD1A <@t> UTHEVS1.mail.uthouston.edu>
Content-Type: text/plain;	charset="us-ascii"

We used to use this formula for general staining with Celestine blue but
came across a formula in the literature many years ago that gave us a
much more intense stain and also lasted a much longer time.
Sorry but cannot remember the reference.

>From memory so hope that this is correct. 
0.5 gms Celestine blue powder into a glass beaker.
Under the fume hood add 0.5 ml of concentrated sulfuric acid and mix
with a glass rod into a paste. During this time the mixture bubbles and
gives off fumes hence the fume hood.
Add 100 ml of 5% aqueous iron alum containing 14 ml of glycerin.
Mix well and filter.
This solution stains very rapidly to start with and staining time
decreases a little after that so you need to test it on sections for
half a minute when it is first made. 
A good way to test whether Celestine blue stains are past their useful
staining life is to swirl the solution around the bottle or flask and if
the solution fails to stick somewhat to the glass then need to make up a
fresh batch. 
Filter each time used.
We really liked this modification as the nuclear stain when using van
Gieson stain as it is not differentiated by the picric acid.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Wednesday, February 08, 2006 8:34 AM
To: 'Julien Lambrey de Souza'; Histonet (E-mail)
Subject: RE: [Histonet] Celestine blue B

Hi,
I started using Celestine Blue in our method for Gomori's Trichrome & it
made a great improvement. I'm not sure why!
Put the slides in Celestine Blue for 2 min. before the Mayer's
Haematoxylin.
Recipe is:
Ferric ammonium sulfate...........5.0 gm
Dissolve in distilled water.......100 ml.
Celestine blue....................0.5 gm
Boil for 3 min. to dissolve, filter, when cooled add-
Glycerin..........................14 ml
Stable for at least 6 months
(Carleton's Histological Techniques 4th edition  P.133)
Hope this helps.
Sharon
sallen <@t> hsc.mb.ca 
-----Original Message-----

This e-mail and/or any documents in this transmission is intended for
the address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission
in error, please notify the sender immediately and return the original.

------------------------------

Message: 8
Date: Wed, 08 Feb 2006 09:47:36 -0600
From: "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>
Subject: Fwd: Re: [Histonet] Alcohol Disposal
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
	<6.0.1.1.0.20060208094734.01a81e48 <@t> mailhost.vetmed.auburn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

>Date: Wed, 08 Feb 2006 09:47:25 -0600
>To: Samuel.Jones2 <@t> med.va.gov 
>From: "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>
>Subject: Re: [Histonet] Alcohol Disposal
>
>
>Hello, our campus' Risk Management Department picks up our waste Alcohol >along with other chemical waste. I'm not sure of their means of disposal. >Best wishes. Atoska
>
>
>At 09:08 AM 2/8/2006, you wrote:
>>
>>
>>Hello All:
>>
>>
>>
>>I've been asked by my peers to see how labs are collecting and disposing of
>>their waste alcohol. Thoughts please. Thanks
>>
>>
>>
>>Samuel E. Jones, MS, HT(ASCP)HTL, QIHC
>>
>>Supervisor, Anatomic Pathology
>>
>>VA North Texas Health Care System
>>
>>4500 South Lancaster Road, 113
>>
>>Dallas, Texas 75216
>>
>>Phone: 214-857-0659
>>
>>e-mail: samuel.jones2 <@t> med.va.gov <mailto:samuel.jones2 <@t> med.va.gov>
>>
>>
>>
>>_______________________________________________dd 0
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu 
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 9
Date: Wed, 8 Feb 2006 08:18:09 -0800 (PST)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] backup cryostat
To: Histonet <@t> lists.utsouthwestern.edu 
Message-ID: <20060208161809.48345.qmail <@t> web90208.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Our company is looking for a backup cryostat.

  Steve

---------------------------------
Relax. Yahoo! Mail virus scanning helps detect nasty viruses!

------------------------------

Message: 10
Date: Wed, 8 Feb 2006 10:39:25 -0600
From: "Malcolm McCallum" <Malcolm.McCallum <@t> tamut.edu>
Subject: [Histonet] infrared vs uv in sequencing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EFF133CED606184A8FC9A6FBBF46714E01AC5887 <@t> STAN.tamut.local>
Content-Type: text/plain;	charset="iso-8859-1"

anyone familiar with the advantages/disadvantages?

Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Till, Renee
Sent: Wed 2/8/2006 8:43 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] argyrophil and argentaffin

I had heard that if you are doing a Grimelius for argyrophil cells, that
an argentaffin stain should also be performed to be able to
differentiate between the two cells. Is this true for any argyrophil
stain?

Renee' Till, HT

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR

72202

================================================================================================

Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
================================================================================================
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 11
Date: Wed, 8 Feb 2006 08:52:15 -0800 (PST)
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] Alcohol Disposal--a few choices
To: samuel.jones2 <@t> med.va.gov, histonet <@t> lists.utsouthwestern.edu 
Message-ID: <20060208165216.21889.qmail <@t> web50902.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Samuel-

  This kind of issue usually starts with the water department in your district.  Some places have enough water going through their system that this kind of waste can go down the drain.  It is considered safe and cost effective ($0!!) because of the dilution factors. Hospitals use HUGE quantities of water so they might even make an exception if it's not in the base policies.  Before you contact your water district, talk with your risk management and environmental services people--they may already know or might want to talk to the water people for you.  

  If you end up not being able to dump it, (and the water company can usually answer you within a few days) you may want to consider recycling.  BR and one other company (??) can do a cost analysis with just a few questions to determine if this won't SAVE your department a buncha bucks (including your people's time in the equasion.)  These systems are SO effective the end result is sometimes better than the original solutions you got from your suppliers.  You can do just alcohols or both Alc. and Xylenes.  These systems are infinitely more useable and safer than they were 20 years ago. 

  The most expensive options are usually waste haulers and they will charge you more for certain contaminants.  Xylene, biologicals, etc.  So when you get a quote from a waste hauler or incinerator, ask if they are going to recycle it themselves (should get you a lower price) or burn it, and any additional cost for other cross-contaminants from your stain line or processors. Being a tree-hugger from way back, I always chose the recycle method when it is fiscally responsible (and it usually is).

  Hope this helps!

  Cheryl Kerry, HT(ASCP)
  Full Staff Inc.
  Staffing the AP Lab by helping one tech at a time.
  admin <@t> fullstaff.org 
  281.852.9457 o
  281.883.7704 c

"Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu> wrote:

>Date: Wed, 08 Feb 2006 09:47:25 -0600
>To: Samuel.Jones2 <@t> med.va.gov 
>From: "Atoska S. Gentry" 
>Subject: Re: [Histonet] Alcohol Disposal
>
>
>Hello, our campus' Risk Management Department picks up our waste Alcohol >along with other chemical waste. I'm not sure of their means of disposal. >Best wishes. Atoska
>
>
>At 09:08 AM 2/8/2006, you wrote:
>>
>>
>>Hello All:
>>
>>
>>
>>I've been asked by my peers to see how labs are collecting and disposing of
>>their waste alcohol. Thoughts please. Thanks
>>
>>
>>
>>Samuel E. Jones, MS, HT(ASCP)HTL, QIHC
>>
>>Supervisor, Anatomic Pathology
>>
>>VA North Texas Health Care System
>>
>>4500 South Lancaster Road, 113
>>
>>Dallas, Texas 75216
>>
>>Phone: 214-857-0659
>>
>>e-mail: samuel.jones2 <@t> med.va.gov 
>>
>>
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu 
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 12
Date: Wed, 08 Feb 2006 11:51:38 CST
From: meint002 <meint002 <@t> umn.edu>
Subject: [Histonet] request feedback on benchtop tissue processors
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <200602081751.k18HpcSv018541 <@t> vanguard.software.umn.edu>
Content-Type: TEXT/plain; CHARSET=US-ASCII

I have been newly hired to do tissue research concerning muscular dystrophy
and genetic cases.  The lab wants to purchase a tissue processor and since
the sample volume would be low, we are looking a benchtop model.  So far I
have read up about the following models; Leica TP1020, Sakura Tissue Tek VIP
150, TBS ATP-120 by Triangle Biomedical Sciences, and STP 120 spin tissue
processors.  Please let me know if you either have one of these models and
what you like or dislike about it.  I would appreciate all feedback you can
provide.

We are also looking into the purchase of a low volume stainer for
immunohistochemical staining.  I would appreciate any feedback on these
models as well.
Thanks very much.

Joyce Meints
Histologist

University of Minnesota
Paul and Sheila Wellstone Muscular Dystrophy Center
MMC 206
420 Delaware St. SE
Minneapolis, MN 55455-0392

e-mail:    meint002 <@t> umn.edu 
lab phone: 612-626-4703

------------------------------

Message: 13
Date: Wed, 8 Feb 2006 09:56:10 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] request feedback on benchtop tissue processors
To: meint002 <meint002 <@t> umn.edu>, histonet <@t> lists.utsouthwestern.edu 
Message-ID: <20060208175610.34431.qmail <@t> web61213.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Joyce:
  According with my experience Sakura tissue procesors are the best. By the way it does not matter if they are benchtop or "self standing", both have the same capacity. One model or the other will depend on your bench top availability.
  Ren* J.

meint002 <meint002 <@t> umn.edu> wrote:
  I have been newly hired to do tissue research concerning muscular dystrophy
and genetic cases. The lab wants to purchase a tissue processor and since
the sample volume would be low, we are looking a benchtop model. So far I
have read up about the following models; Leica TP1020, Sakura Tissue Tek VIP
150, TBS ATP-120 by Triangle Biomedical Sciences, and STP 120 spin tissue
processors. Please let me know if you either have one of these models and
what you like or dislike about it. I would appreciate all feedback you can
provide.

We are also looking into the purchase of a low volume stainer for
immunohistochemical staining. I would appreciate any feedback on these
models as well.
Thanks very much.

Joyce Meints
Histologist

University of Minnesota
Paul and Sheila Wellstone Muscular Dystrophy Center
MMC 206
420 Delaware St. SE
Minneapolis, MN 55455-0392

e-mail: meint002 <@t> umn.edu 
lab phone: 612-626-4703

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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