[Histonet] Celestine blue B

[Sharon Allen]

Hi,
I started using Celestine Blue in our method for Gomori's Trichrome & it
made a great improvement. I'm not sure why!
Put the slides in Celestine Blue for 2 min. before the Mayer's Haematoxylin.
Recipe is:
Ferric ammonium sulfate...........5.0 gm
Dissolve in distilled water.......100 ml.
Celestine blue....................0.5 gm
Boil for 3 min. to dissolve, filter, when cooled add-
Glycerin..........................14 ml
Stable for at least 6 months
(Carleton's Histological Techniques 4th edition  P.133)
Hope this helps.
Sharon
sallen <@t> hsc.mb.ca
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