[Histonet] Re-processing (Johnson)

[Tony Henwood]

We use Leica carousel processors.
I would not expect too much wax carryover in the VIP using this method

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Saturday, 4 February 2006 5:40 AM
To: Histonetliste (Histonetliste)
Subject: AW: [Histonet] Re-processing (Johnson)


 
What kind of processor is used with this method?
We have a VIP and were instructed to take care of too much paraffin in the container when starting the run (particels left after cleaning). So it sounds very easy to reprocess the tissue, but I don't want to break down the machine.

Gudrun Lang



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Tony Henwood
Gesendet: Donnerstag, 02. Februar 2006 23:23
An: Celebre Julia; Histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Re-processing 

The following technique from Johnson (2003) Histologic 36(1):21-22 works very well:

While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. I have often had recourse to use this method and have found it to dramatically improve the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003).


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
Sent: Friday, 3 February 2006 4:21 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re-processing 


Calling all processing and reprocessing experts out there....

I'm sure all labs over the years have run into processing troubles ranging from instrument malfunctions during the night to misplacement of solutions (usually a water before the xylene), causing pure horror for the technologist who opens up the retort in the morning.  It's sad to say that our department is experienced in these situations, and everytime something happens to the processing it's always difficult to decide how best to reprocess the sections. 

Each time we've reprocessed our sections (usually due to the introduction of water before the xylene or paraffin stations) on shortened absolute alcohol, xylenes and paraffin times, they'v ended up being extremely hard and brittle, which does not make our pathologist happy....

Is there a way of reprocessing tissue without damaging it? 
Would adding a softener to the alcohols reduce the hardening affects of reprocessing? How much time can be safely shaved off if the tissues have already been through a regular program? 

Any help is greatly appreciated,

Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
Hamilton, Ontario
Canada
905-527-0271  ext 46179
email: celebrej <@t> hhsc.ca




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