[Histonet] Re: overprocessing

[carlos]

Hi Kemlo,
I must disagree re hardening......if only in my experience of the following: 
I have processed gelatin ( 1%)blocks after formalin fixation (  1cm blocks 
fixed for 2, 5, 10 and 60 days) and find that they all suffer from shrinkage 
and hardening. My processing is via gradual alcohols to alc/xylene 1:1, then 
xylene x2 and wax x2. Two hrs in each.
Be grateful for enlightenment.
Best wishes
carl