Hardness of tissues RE: [Histonet] processing

[Pamela Marcum]

Ada Feldman also discusses bound water in one of her lectures.  I think it 
is the one about Histology and the Kitchen.  I believe Anatech also did a 
newsletter or flyer with some information on bound water and 
processing/dehydration etc.  Ethel Macrea has also discussed it in the 
past.  It was very interesting and helpful.   Animals do appear to be more 
lean and the lack of fat or lipid content can effect processing.  It is why 
we in the VIR area have very different protocols from those doing human 
work routinely.  We see it here all the time.
No Andi you are not making it up and it would be very helpful if someone 
did more on this subject.

Pam Marcum

At 10:39 AM 2/3/2006, Gayle Callis wrote:
>Not an intellectual battle, just a discussion.
>
>I would like to share this message from a lady has vast experience 
>working  animal tissues, sent yesterday.  NOT all animal tissues, 
>obviously, are going to have less % of fat, but some are worse than others.
>
>>Jerry Fredenburg did a wonderful workshop back in the dark ages about 20 
>>yrs ago on the chemistry of processing and staining and talked about 
>>bound water. Funny you should mention it because I was discussing this 
>>with my student only a few days ago. I'm always meaning to dig out that 
>>handout where he explained it so well. They always think I'm making up 
>>the term "bound water".
>>I think the animals eat healthier and there are less lipids in their 
>>tissue. When I get rodents who were given a fatty diet their tissue are 
>>not so dried out. Their livers are dripping with Oil Red O when I do that 
>>stain on frozens - so much that sometimes coverslipping pushes out the 
>>red stained lipids.
>>
>>Andi
>At 01:53 AM 2/3/2006, you wrote:
>>The hardness caused by processing is presently an intellectual battle
>>between myself and Gayle (covert to this List).
>>
>>I'm presently in think mode on this.
>>
>>Kemlo Rogerson
>>Pathology Manager
>>Ext  3311
>>DD   01934 647057
>>Mob 07749 754194
>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
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Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu