[Histonet] MSH6

[Orr, Rebecca]

Hi Josie,
How many different types of protocols did you try on the XT?
With the different temp and Cell conditioning options I'm surprised you
didn't get something.
Are you running the MLH-1 and MSH-2 as well?  How are they?

My experience with the Microsateliite Instabilities is 4+ staining in 2
out of 3 antibodies, depending on the control tissue.  The third
antibody would compare at 2+ or a bit stronger.

I have the best of both worlds with a Benchmark and a Nemesis.  I
experienced the poor staining results of these particular antibodies and
I decided to run them on the Nemesis with Biocare polymer detection and
they are gorgeous. I use the Biocare antibodies, too.
In case you don't have that option, I would consider trying another
control tissue, and running through all the CC options.   I'm not sure
if they behave differently without heat, did you try running a protocol
with out heat in the detection? 
Do you have access to the polymer from Ventana?  Maybe try that?  Or run
the antibody at 1:5 or 1:10.
Or do you have a pressure cooker to try the HEIR offline.  If you get
good staining with offline HEIR then you can rule out the detection as
the issue, too.

Becky

 Becky Orr CLA,HT(ASCP)
IHC Lead 
Evanston Northwestern Healthcare
847-570-2771
 
-----Original Message-----


Message: 8
Date: Wed, 1 Feb 2006 13:54:36 -0600
From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
Subject: [Histonet] looking for Antibodies-MSH6  and PMS2
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<2C515C1049EAF5459EFD8C9B929078A41945A8 <@t> phdex03.txhealth.org>
Content-Type: text/plain;	charset="us-ascii"

Hello Everyone ,
I am using Ventana BMK/XT , can someone tell me a good source of MSH6
and PMS2  that will work  on the Ventana  Machine. I  appreciate  any
information. Thank you.
 
Josie