[Histonet] MGMT

[Elaine Dooley]

Dear Histonetters,

I have been trying to get the antibody MGMT (
O6-Methylguanine-DNA-Methyltransferase) to work on a Ventana Benchmark
system.  I have one control that appeared to work but I have not found
any other tissue that has stained.  I feel I need to tweak this antibody
some perhaps I am getting false negative reactions.  I have been using
Lab Vision Neomarkers antibody at 1:50 with mild CC1 epitope retrieval
with amplifier.  (using i-view dab detection)

Any comments may be helpful

Elaine Dooley
HTL
Shands Teaching Hosp.
Gainesville FL 
352-265-0111 ext 72117