[Histonet] fall off sections

[Rene J Buesa]

Jonathan:
  Usually (I repeat usually) a section falls after IHC if the tissue has had some processing problems and it was not completely infiltrated, usually also because there was a problem with fixation.
  Under those circumstances, also usually the section is "microscopically uneven", meaning that it cannot adhere properly and completely to the slide and begins to microscopically peel-off or to separate from the glass until it finally falls-off completely.
  Even if you did not experience this problem before, perhaps because of any problem with processing, it now is happening.
  Check your processing protocol and its QA: have there been any changes? New reagents manufacturer? A new operator? Change in some procesing times? Same fixation period? Same source of the specimens? Any of these factors could influence.
  If you have not changed the IHC protocol at all, the cause has to be in the "pre-IHC" steps.
  Hope this will help you!
  René J. 

Jonathan De La Rosa <jdhisto <@t> yahoo.com> wrote:
  HELLO,

I'M HAVING A SECTION (BREAST) THAT HAS FALLEN OFF SEVERAL TIMES AFTER IHC RUN. THIS HAS BEEN ATTEMPTED 4 TIMES AND IS THE FIRST TIME IVE EXPERIENCED IT TO THIS DEPT. ANY SUGGESTIONS ?


Jonathan N. De La Rosa HT
New Braunfels, TX 78130
210-412-5837
JDhisto <@t> yahoo.com

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