[Histonet] Von Kossa stain

Gayle Callis gcallis <@t> montana.edu
Mon Dec 18 09:37:31 CST 2006

Toluidine Blue, Sterchi method, or MacNeals Tetracrhome (in house recipe - 
we found the commercial solution did not work well) - I will be happy to 
foward methods if you wish.

When you do basic fuchsin, you need to cut back on concentration of the 
basic fuchsin and also the time of staining.  Take on section and play with 
the timing/dye concentration.

At 01:25 AM 12/18/2006, you wrote:
>Hello everybody,
>we work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and 
>embedded in methylmethacrilate (MMA).
> >From our samples we obtain 50 µm thick sections which can contain 
> titanium implants.
>We tried to perform Von Kossa stain, always on undeplasticized sections, 
>but we met several problems:
>- calcium deposits after silver nitrate+UV light did not appear black but 
>brown, and, after sodium thiosulphate, brown stain turns more and more light;
>- we could not find a suitable counterstain for osteoid: nuclear fast red 
>and neutral red do not stain at all, basic or acid fuchsin stain too much 
>and cover the underlying brown..
>Some suggestions? Are there other good histological stainings for 
>undeplasticized bone sections in your experience?
>Thank you!
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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