[Histonet] Methylmethacrylate to LR White?

[Pamela Marcum]

Patsy is correct GMA can not be removed and it is part of the LR 
White formula.  Pam Marcum

At 11:18 AM 12/15/2006, pruegg <@t> ihctech.net wrote:
>Matt,
>Methyl methacrylate can be removed with xylene, but glycol methacrylate can
>not ever be completely removed, I believe LR White is a glycol methacrylate.
>Patsy
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of matt
>prideaux (RI)
>Sent: Friday, December 15, 2006 5:02 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Methylmethacrylate to LR White?
>
>Hi all,
>
>I need to remove the methacrylate surrounding some bone samples (I was
>told that a 50:50 solution of xylene and chloroform would do the trick).
>The problem is that I want to re-embed the samples in LR White once the
>methacrylate has been removed. Does anyone have any experience in this
>or knows if it is possible? I need to cut 10 micron thick cross-sections
>of mouse tibiae and these have failed to adequately infiltrate with
>methacrylate. With LR White being less viscous I was hoping that it
>would infiltrate better.
>
>Thanks
>
>Matt
>
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Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu