[Histonet] paraffin problem
barbara.bublava <@t> meduniwien.ac.at
Thu Dec 14 06:17:50 CST 2006
Du you turn off your embedding-tank between usage?
I did have similar problems when I used to do that.
Seems like paraffin does not like repeated melting and hardening.
Since I keep paraffin melted this problem does not appear anymore.
I also stirr it bevore embedding.
Vickroy, Jim wrote:
> Lately we are experiencing a problem with our paraffin blocks. We use
> Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon
> very well and at times the sections have some small striations, almost
> like knife lines. We have checked the disposable knives and have
> eliminated that variable. We use a recycled clearing agent but have
> eliminated that also by using fresh chemicals. We have several tissue
> processors and there is a possibility that the problem could be a
> processor issue however the majority of us think that it is a paraffin
> problem. We do not see any "grit" in the paraffins. We are thinking of
> trying a different paraffin and wondered if anyone has switched to one
> of the newer paraffins that work great. Obviously I can get as many
> samples as I would like but want to see if anyone has any suggestions or
> ideas that would help us eliminate some of the steps of the process.
> Jim Vickroy
> Technical Supervisor - Surgical and Autopsy Pathology
> Memorial Medical Center
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