[Histonet] RE: Polymer Feedback?
Margaryan, Naira
NMargaryan <@t> childrensmemorial.org
Mon Dec 11 16:42:36 CST 2006
Hello,
I never used polymer. My questions are:
1. Is it has to melt as paraffin, if so what degree?
2. What is the depolymerization protocol?
3. Is it requires Antigen retrieval step?
4. Why it's not requires Avidin/Biotin step?
Thank you,
Naira
Naira V. Margaryan, D.V.M., Ph.D.
Research Associate III
Children's Memorial Research Center
2300 Children's Plaza, Box 222
Chicago, IL 60614-3394
Tel: 773-755-6570/ext-8
Fax: 773-755-6594
nmargaryan <@t> childrensmemorial.org
For Express Mail:
CMRC, Room C.473
2430 N. Halsted Street
Chicago, IL 60614-4314
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, December 08, 2006 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 37, Issue 9
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Today's Topics:
1. Automated stainer (Johnson, Teri) (Jerry Helisek)
2. RE: Polymer Feedback? (Dawson, Glen)
3. Re: paraffin problem (rsrichmond <@t> aol.com)
4. RE: Polymer Feedback? (Bonnie Whitaker)
5. RE: Polymer Feedback? (Horn, Hazel V)
6. looking for someone to do a Brucella suis IHC on Caribou (HCS)
7. looking for a slide carrier for Shandon X-Y stainer (HCS)
8. RE: Polymer Feedback? (patsy ruegg)
9. RE: Re: paraffin problem (patsy ruegg)
10. RE: Polymer Feedback? (patsy ruegg)
11. Shur/mark slides (Kimberly Johnson)
12. GFP and B-Gal positive control slides (Rebecca Nishi)
13. RE: GFP and B-Gal positive control slides (Koelling, Ray)
14. Fading (histotech <@t> charter.net)
15. RE: neurospheres (Tarango, Mark)
16. ICAM-1 Antibody (Wulan Anggrahini)
17. cryostat whisperer???... (i need parts for a Leica, STAT)
(Debbie Keith)
18. RE: Fading (Kemlo Rogerson)
19. Re: Fading (Rene J Buesa)
20. Microscope slide trays -- not folders (Victoria Baker)
21. RE: Microscope slide trays -- not folders (Horn, Hazel V)
22. cryostat whisperer???... (i need parts for a Leica, STAT)
(Stephen Peters M.D.)
23. RE: Shur/mark slides (Kellar, Eric C)
24. employment (Bonnie Whitaker)
25. CD 79 antibody (Colin Nixon)
26. Re: CD 79 antibody (Rene J Buesa)
27. digital image analysis systems... (Breckenridge, Richard A)
----------------------------------------------------------------------
Message: 1
Date: Thu, 7 Dec 2006 13:22:16 -0500
From: "Jerry Helisek" <Jerry <@t> ralambusa.com>
Subject: [Histonet] Automated stainer (Johnson, Teri)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3855F92002259948A66A8CA2D16E3A4F017BB3 <@t> server.ralambusa.com>
Content-Type: text/plain; charset="us-ascii"
Teri,
Please see:
http://www.ralamb.net/product_info.php?products_id=595&osCsid=52e93ef809
10bcc854ff5818dc168ef6
I think this may be what you are looking for in a small stainer (RA
Lamb's StainMate)
Jerry Helisek
5409 Lumley Road, Unit #102
Durham, North Carolina 27703
Phone: 919-957-1964
Fax: 919-957-1972
Cell: 919-264-7964
jerry <@t> ralambusa.com
www.ralamb.com
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Message: 2
Date: Thu, 7 Dec 2006 12:24:51 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] Polymer Feedback?
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B3D65550856D0146B900D401EE313D4B6AAA7F <@t> dynams.dynacaremilwaukee.com>
Content-Type: text/plain; charset="iso-8859-1"
Doug,
Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on.
Only bad thing is the cost but nothing is perfect.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Doug
Geddes
Sent: Thursday, December 07, 2006 11:48 AM
To: Histonet <@t> lists.utsouthwestern.edu; Gayle Callis
Subject: Re: [Histonet] Polymer Feedback?
Sorry, polymers for IHC staining.
Doug
>>> Gayle Callis <gcallis <@t> montana.edu> 2006-12-07 12:36 PM >>>
Doug,
Polymers for what purpose, embedding tissues?
At 09:22 AM 12/7/2006, you wrote:
>Dear Histonet,
>
>Looking for feedback and experiences with different ploymers
>(preferably universal) in a clinical setting.
>
>Doug Geddes BSc, MLT
>Dept of Pathology
>LHSC London ON
>Canada
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
-----------------------------------------
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Message: 3
Date: Thu, 07 Dec 2006 13:36:53 -0500
From: rsrichmond <@t> aol.com
Subject: [Histonet] Re: paraffin problem
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C8E845CD1DB9FA-250-41C1 <@t> MBLK-M23.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Several people comment on problems with present day embedding waxes ("paraffin").
Geezer time. In the more than forty years I've been a pathologist, one of the biggest improvements in technology has been the replacement of simple paraffin waxes with the present proprietary mixtures. In the 1960's, many laboratories simply bought Gulfwax - the wax used by home canners to seal canning jars. For quite a few years now complex mixtures of who knows what have replaced simple paraffin. These mixtures are all proprietary and trade-secret, and as far as I know this whole change has been largely undocumented in the literature. But the improvement in sections that has resulted has been revolutionary. In the 1960's many laboratories were simply unable to cut an interpretable section of a lymph node. Frank Foote, then the chief of surgical pathology at Memorial/Sloan Kettering in New York city, often observed that most of a surgical pathology consultant's expertise was in interpreting nearly unreadable slides.
Bob Richmond
Samurai Pathologist
Knoxville TN
________________________________________________________________________
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------------------------------
Message: 4
Date: Thu, 7 Dec 2006 13:06:39 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: RE: [Histonet] Polymer Feedback?
To: "'Dawson, Glen'" <GDawson <@t> dynacaremilwaukee.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000a01c71a32$d30055d0$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain; charset="us-ascii"
Like a boss used to tell me: You can have better quality, you can have a
faster turnaround time or you can produce a product that is cheaper. Pick
2. You can never have all three!
Polymers are faster and better (my favorite pick for the 2)
Bonnie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Thursday, December 07, 2006 12:25 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polymer Feedback?
Doug,
Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save
some of their extra cost by taking antibody titers out farther. Clean.
Fewer steps. So on and so on.
Only bad thing is the cost but nothing is perfect.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Doug Geddes
Sent: Thursday, December 07, 2006 11:48 AM
To: Histonet <@t> lists.utsouthwestern.edu; Gayle Callis
Subject: Re: [Histonet] Polymer Feedback?
Sorry, polymers for IHC staining.
Doug
>>> Gayle Callis <gcallis <@t> montana.edu> 2006-12-07 12:36 PM >>>
Doug,
Polymers for what purpose, embedding tissues?
At 09:22 AM 12/7/2006, you wrote:
>Dear Histonet,
>
>Looking for feedback and experiences with different ploymers
>(preferably universal) in a clinical setting.
>
>Doug Geddes BSc, MLT
>Dept of Pathology
>LHSC London ON
>Canada
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
-----------------------------------------
This information is directed in confidence solely to the person named above
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------------------------------
Message: 5
Date: Thu, 7 Dec 2006 13:45:07 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Polymer Feedback?
To: "Doug Geddes" <Doug.Geddes <@t> lhsc.on.ca>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6EFF <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii
I'm interested in polymers as well. This is new for me.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Doug
Geddes
Sent: Thursday, December 07, 2006 10:23 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Polymer Feedback?
Dear Histonet,
Looking for feedback and experiences with different ploymers
(preferably universal) in a clinical setting.
Doug Geddes BSc, MLT
Dept of Pathology
LHSC London ON
Canada
-----------------------------------------
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Message: 6
Date: Thu, 7 Dec 2006 12:27:13 -0800
From: "HCS" <int09018 <@t> alphahunt.com>
Subject: [Histonet] looking for someone to do a Brucella suis IHC on
Caribou
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c71a3e$16ca4e00$6701a8c0 <@t> hp>
Content-Type: text/plain; charset="iso-8859-1"
Hi, I would like to find someone who does IHC that could do a Brucella suis stain on a Caribou. Please email me if you do this stain or know of a contact for me.
Thanks
LeRoy Brown HT(ASCP) HTL
HCS, Inc.
360-966-7300
------------------------------
Message: 7
Date: Thu, 7 Dec 2006 12:29:14 -0800
From: "HCS" <int09018 <@t> alphahunt.com>
Subject: [Histonet] looking for a slide carrier for Shandon X-Y
stainer
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c71a3e$5cf1e640$6701a8c0 <@t> hp>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have an extra slide carrier that fits the Shandon x-y stainer?
thanks
LeRoy Brown Ht(ASCP) Htl
HCS, Inc.
360-966-7300
------------------------------
Message: 8
Date: Thu, 7 Dec 2006 13:54:10 -0700
From: "patsy ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Polymer Feedback?
To: "'Bonnie Whitaker'" <bwhitaker <@t> brownpathology.com>, "'Dawson,
Glen'" <GDawson <@t> dynacaremilwaukee.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200612072054.kB7KsBen082197 <@t> pro12.abac.com>
Content-Type: text/plain; charset="us-ascii"
Bonnie,
You are generous, my boss always said "pick one" out of the three you
mentioned.
In the case of labeled polymer I think you are right, they are faster and I
think better, at least I do not have to worry about endogenous biotin
issues. They an't cheap though.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bonnie
Whitaker
Sent: Thursday, December 07, 2006 12:07 PM
To: 'Dawson, Glen'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polymer Feedback?
Like a boss used to tell me: You can have better quality, you can have a
faster turnaround time or you can produce a product that is cheaper. Pick
2. You can never have all three!
Polymers are faster and better (my favorite pick for the 2)
Bonnie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Thursday, December 07, 2006 12:25 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polymer Feedback?
Doug,
Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save
some of their extra cost by taking antibody titers out farther. Clean.
Fewer steps. So on and so on.
Only bad thing is the cost but nothing is perfect.
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Doug Geddes
Sent: Thursday, December 07, 2006 11:48 AM
To: Histonet <@t> lists.utsouthwestern.edu; Gayle Callis
Subject: Re: [Histonet] Polymer Feedback?
Sorry, polymers for IHC staining.
Doug
>>> Gayle Callis <gcallis <@t> montana.edu> 2006-12-07 12:36 PM >>>
Doug,
Polymers for what purpose, embedding tissues?
At 09:22 AM 12/7/2006, you wrote:
>Dear Histonet,
>
>Looking for feedback and experiences with different ploymers
>(preferably universal) in a clinical setting.
>
>Doug Geddes BSc, MLT
>Dept of Pathology
>LHSC London ON
>Canada
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
-----------------------------------------
This information is directed in confidence solely to the person named above
and may contain confidential and/or privileged material. This information
may not otherwise be distributed, copied or disclosed. If you have received
this e-mail in error, please notify the sender immediately via a return
e-mail and destroy original message. Thank you for your cooperation.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 9
Date: Thu, 7 Dec 2006 13:57:26 -0700
From: "patsy ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Re: paraffin problem
To: <rsrichmond <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200612072057.kB7KvRp5083970 <@t> pro12.abac.com>
Content-Type: text/plain; charset="us-ascii"
I agree Samurai, the better paraffin and disposable knives have changed
paraffin sectioning forever for better.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rsrichmond <@t> aol.com
Sent: Thursday, December 07, 2006 11:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: paraffin problem
Several people comment on problems with present day embedding waxes
("paraffin").
Geezer time. In the more than forty years I've been a pathologist, one of
the biggest improvements in technology has been the replacement of simple
paraffin waxes with the present proprietary mixtures. In the 1960's, many
laboratories simply bought Gulfwax - the wax used by home canners to seal
canning jars. For quite a few years now complex mixtures of who knows what
have replaced simple paraffin. These mixtures are all proprietary and
trade-secret, and as far as I know this whole change has been largely
undocumented in the literature. But the improvement in sections that has
resulted has been revolutionary. In the 1960's many laboratories were simply
unable to cut an interpretable section of a lymph node. Frank Foote, then
the chief of surgical pathology at Memorial/Sloan Kettering in New York
city, often observed that most of a surgical pathology consultant's
expertise was in interpreting nearly unreadable slides.
Bob Richmond
Samurai Pathologist
Knoxville TN
________________________________________________________________________
Check out the new AOL. Most comprehensive set of free safety and security
tools, free access to millions of high-quality videos from across the web,
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------------------------------
Message: 10
Date: Thu, 7 Dec 2006 13:45:08 -0700
From: "patsy ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Polymer Feedback?
To: "'Doug Geddes'" <Doug.Geddes <@t> lhsc.on.ca>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200612072045.kB7Kj8H3077358 <@t> pro12.abac.com>
Content-Type: text/plain; charset="us-ascii"
Do you mean labeled polymers for ihc?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Doug Geddes
Sent: Thursday, December 07, 2006 9:23 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Polymer Feedback?
Dear Histonet,
Looking for feedback and experiences with different ploymers
(preferably universal) in a clinical setting.
Doug Geddes BSc, MLT
Dept of Pathology
LHSC London ON
Canada
-----------------------------------------
This information is directed in confidence solely to the person
named above and may contain confidential and/or privileged
material. This information may not otherwise be distributed, copied
or disclosed. If you have received this e-mail in error, please
notify the sender immediately via a return e-mail and destroy
original message. Thank you for your cooperation.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 7 Dec 2006 13:09:01 -0800
From: "Kimberly Johnson" <kjohnson <@t> covx.com>
Subject: [Histonet] Shur/mark slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCED920644E00046BEC7D73C484219DC050B1E <@t> covxmailbe.covx.com>
Content-Type: text/plain; charset="us-ascii"
Hello!
I finally got myself an automated slide labeler! YAY! But I need
some help on what slides to use. I have the Shur/mark labeler from TBS,
and got some slides, but I realized I needed to get some positively
charged slides. I know a lot of you use this machine and was wondering
if you all could guide me towards a good slide that is charged that
works with the labeler. Thank you so much for your time, your advise is
always great!
Kim
Kimberly Johnson
Research Associate II
CovX
Ph. (858)964-2050
Fax (858)964-2090
The information contained in this e-mail message may be privileged and
confidential information and is intended only for the use of the
individual and/or entity identified in the alias address of this
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Message: 12
Date: Thu, 7 Dec 2006 13:34:17 -0800
From: Rebecca Nishi <rnishi <@t> uci.edu>
Subject: [Histonet] GFP and B-Gal positive control slides
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4B87DFCC-BE93-449B-85A0-CCF5C15AA67C <@t> uci.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Does anyone know where I can buy, or can suggest an easy way to make
positive control slides for GFP and B-Gal.
We have use AAV contstructs with either GFP or LAC-Z and injected
into mice. We have sectioned the tissue and would like to look for
positive GFP or B-Gal. It would be nice to have a positive control
slide. We plan on visualizing both with and without antibody
amplification. I would appreciate any suggestions.
Thank You.
Rebecca
------------------------------------
Rebecca Nishi
UC Irvine
Christopher Reeve Foundation SCI Core/Anderson Lab
1226 GNRF
Irvine, CA 92697-4540
rnishi <@t> uci.edu
Phone/Fax: 949-824-9728
------------------------------
Message: 13
Date: Thu, 7 Dec 2006 14:05:03 -0800
From: "Koelling, Ray" <koellinr <@t> amgen.com>
Subject: RE: [Histonet] GFP and B-Gal positive control slides
To: Rebecca Nishi <rnishi <@t> uci.edu>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<16834C6DFFA6004C88DE4507FB8AE544059839E9 <@t> wa-mb4-sea.amgen.com>
Content-Type: text/plain
Rebecca,
For GFP, that can be a can of worms. For B-Gal this is what we do. From
mouse vendor, get a Tie-2/lacZ transgenic mouse strain. Are readily
available commercially. Tie-2 is expressed in endothelium and the lacZ
reporter gene is co-expressed there. Take any tissue (kid/liver/whatever).
B-Gal antibodies show beautifully stained endothelium.
Ray
Raymond Koelling
Research Scientist
Amgen Seattle
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Nishi
Sent: Thursday, December 07, 2006 1:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GFP and B-Gal positive control slides
Does anyone know where I can buy, or can suggest an easy way to make
positive control slides for GFP and B-Gal.
We have use AAV contstructs with either GFP or LAC-Z and injected
into mice. We have sectioned the tissue and would like to look for
positive GFP or B-Gal. It would be nice to have a positive control
slide. We plan on visualizing both with and without antibody
amplification. I would appreciate any suggestions.
Thank You.
Rebecca
------------------------------------
Rebecca Nishi
UC Irvine
Christopher Reeve Foundation SCI Core/Anderson Lab
1226 GNRF
Irvine, CA 92697-4540
rnishi <@t> uci.edu
Phone/Fax: 949-824-9728
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 7 Dec 2006 15:59:38 -0800
From: <histotech <@t> charter.net>
Subject: [Histonet] Fading
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1064608487.1165535978746.JavaMail.root <@t> fepweb05>
Content-Type: text/plain; charset=utf-8
Hello,
For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation?
Thank you,
DDietz
Morristown-Hamblen Lab
------------------------------
Message: 15
Date: Thu, 7 Dec 2006 16:38:40 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] neurospheres
To: "Atoska Gentry" <gentras <@t> vetmed.auburn.edu>, "Histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDEE009BE <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii
When I take the chamber/divider thing off the slide, I make sure to
remove the media first (and very carefully so the cells don't come off
the slide). Then I make sure to let it air dry for plenty of time
before I do anything to it. If she is trying to use chamber slides on a
cell culture, I hope they are adherent cells. If they are the kind of
cells that grow in suspension (like hematopoietic cells), then you won't
many cells adhere. I don't know if neurospheres will adhere...
Mark Adam Tarango HT(ASCP)
Histology/Immunohistochemistry Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
mtarango <@t> nvcancer.org
Direct Line (702) 822-5112
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska
Gentry
Sent: Thursday, December 07, 2006 8:19 AM
To: Histonet
Subject: [Histonet] neurospheres
Hello, does anyone have a protocol for fixation & staining neurospheres
from tissue culture either on chamber slides or cryosections? If so will
you be so kind as to share it with me ASAP? One of our researchers here
has attempted using the chamber slides but, she said the cells all
washed off. Technique / protocol suggestions will be much appreciated.
Thanks, Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
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"EMF <nvcancer.org>" made the following annotations.
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Message: 16
Date: Fri, 8 Dec 2006 10:45:21 +0900
From: "Wulan Anggrahini" <wulan <@t> med.kobe-u.ac.jp>
Subject: [Histonet] ICAM-1 Antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001a01c71a6a$85cc4200$0bc8a8c0 <@t> your56ab121f6e>
Content-Type: text/plain; charset="iso-8859-1"
Hi..
I need information about good purified rat anti mouse ICAM-1 monoclonal antibody. I want to buy from Chemicon but there are apparently two types of clones from Chemicon for rat antimouse ICAM-1. CLone KAT-1 and Clone LTF653. Does anyone has any experience on using this antibody ?
I need to stain mouse vascular tissue on frozen section with 4% Paraformaldehyde fixation. Thanks a lot for the help.
Regards,
Wulan Anggrahini
Kobe University
------------------------------
Message: 17
Date: Thu, 07 Dec 2006 20:11:44 -0700
From: Debbie Keith <debbiekeith <@t> cox.net>
Subject: [Histonet] cryostat whisperer???... (i need parts for a
Leica, STAT)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.2.0.9.0.20061207195737.00ba7908 <@t> pop.central.cox.net>
Content-Type: text/plain; charset=us-ascii; format=flowed;
x-avg-checked=avg-ok-3C9AC07
i'd like to thank everyone for their input about my recent cryostat issues...
the biomedical repair-folk came out on wednesday and diagnosed the
issue. it seems the screw/lever on the chuck assembly (that tightens the
chuck) has been over-tightened to the point of stripping the threads and
rendering it chronically "un-tight". the harder it's tightened... the
harder it has to be tightened.
one of the repair-folk actually had what MUST have been a "Viola
Moment". he tightened the handle and it sectioned like a spanky-new
machine would. it was beautiful. after he left, i cut the next block...
and had to adjust the chuck. keep in mind that this repair guy is a
STRAPPING person that could EASILY haul large farm equipment with the best
of them.... and i have the upper body strength of the average 3 year-old.
after much crying, causing ice crystals to form and a hush to fall... i
called the repair guy. he told me it was going to take 2-4 weeks for the
parts to come from Leica.
is there anyone out there that KNOWS where to get the parts quicker?
i would like to note... the repair guy is pretty good. he doesn't make
much conversation... but he "listens" to the machine. he said, without
even looking at the machine, "i listen for the sound the machine makes when
it's cutting 'right'."
later, when it WAS cutting right... he said "do you hear that?? huh? do you
hear THAT???". it DID make a very distinct sound. next time you make a
beautiful section... listen for the "sssschhhuctt" sound. THEN, think of
me crying into my cm1510.
(seriously, any ideas about parts?)
debbie
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------------------------------
Message: 18
Date: Fri, 8 Dec 2006 08:22:36 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Fading
To: <histotech <@t> charter.net>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<86ADE4EB583CE64799A9924684A0FBBF01246F79 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Would be useful to know which stain is fading. I assume H&E and in that
case which element, the 'H' or the 'E'?
Causes are different.
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
Pager 07659 597107
E-Mail: kemlo.rogerson <@t> nhs.net
Once you are in the orbit of your destiny, weightlessness is the only
result. --Baba Amte
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Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
------------------------------
Message: 19
Date: Fri, 8 Dec 2006 05:29:50 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Fading
To: histotech <@t> charter.net, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <747142.68162.qm <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Even when theoretically after dehydration is completed all the water should have been removed from the section, IF the tap water used after the hamoxylin is "reach" in chlorine, those ions will react with the dyes and will make them to fave even after the sections have been coverslipped.
Check for chlorine in your tap water supply.
René J.
histotech <@t> charter.net wrote:
Hello,
For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation?
Thank you,
DDietz
Morristown-Hamblen Lab
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------------------------------
Message: 20
Date: Fri, 8 Dec 2006 09:29:10 -0500
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: [Histonet] Microscope slide trays -- not folders
To: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
Message-ID:
<4f016b690612080629h1f035e45od6501842298f395 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Happy Friday Everyone!
Thanks to everyone who responded to my e-mail about American Histo
Labs, it was a big help.
Now, I'm searching for slide trays that are about 14"long and 11-1/2"
wide. Some are wood others are an acrylic I think. They have 4 rows
and hold a lot of slides. I've been trying to find them through my
usual sources without success.
Any information is welcomed.
Have a nice weekend.
Vikki
------------------------------
Message: 21
Date: Fri, 8 Dec 2006 09:07:53 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Microscope slide trays -- not folders
To: "Victoria Baker" <bakevictoria <@t> gmail.com>, "Histo Net list server"
<HistoNet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F01 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii
Try www.brainlab.com
They have them.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Friday, December 08, 2006 8:29 AM
To: Histo Net list server
Subject: [Histonet] Microscope slide trays -- not folders
Happy Friday Everyone!
Thanks to everyone who responded to my e-mail about American Histo
Labs, it was a big help.
Now, I'm searching for slide trays that are about 14"long and 11-1/2"
wide. Some are wood others are an acrylic I think. They have 4 rows
and hold a lot of slides. I've been trying to find them through my
usual sources without success.
Any information is welcomed.
Have a nice weekend.
Vikki
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 22
Date: Fri, 8 Dec 2006 07:18:58 -0800 (PST)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] cryostat whisperer???... (i need parts for a
Leica, STAT)
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <466458.27471.qm <@t> web30404.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Debbie,
Try my friends at Belair Instrument Company. 973 912 8900.
They sell and service these and I expect they would have something in stock to help you.
Stephen
Stephen Peters M.D.
Vice Chairman of Pathology
Hackensack University Medical Center
201 996 4836
Pathology Innovations, LLC
410 Old Mill Lane,
Wyckoff, NJ 07481
201 847 7600
www.pathologyinnovations.com
------------------------------
Message: 23
Date: Fri, 8 Dec 2006 10:35:52 -0500
From: "Kellar, Eric C" <Eric.C.Kellar <@t> questdiagnostics.com>
Subject: RE: [Histonet] Shur/mark slides
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6843061CE6B98E4B96590D4F299618F801583BF0 <@t> qdcws0117.us.qdx.com>
Content-Type: text/plain; charset=iso-8859-1
Kim,
I have two Shur/mark slides etchers that run all day long. Most Colorfrost Plus slides in darker colors - blue/green/tan available from Cardinal, Thermo Fisher, Lamb all work well. I have also tried Shur/mark brand Colorfrost Plus slides, they come with a color painted background on the opposite side of the slide to enhance visibility. They are very nice, but are extremely overpriced. It's the diamond stylus that needs to be replaced often with any frosted slide you choose. They're expensive too and come in a five pack, so stock up.
Eric C. Kellar
Quest Diagnostics, Inc
Histology Laboratory Supervisor
Miami
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimberly Johnson
Sent: Thursday, December 07, 2006 4:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Shur/mark slides
Hello!
I finally got myself an automated slide labeler! YAY! But I need
some help on what slides to use. I have the Shur/mark labeler from TBS,
and got some slides, but I realized I needed to get some positively
charged slides. I know a lot of you use this machine and was wondering
if you all could guide me towards a good slide that is charged that
works with the labeler. Thank you so much for your time, your advise is
always great!
Kim
Kimberly Johnson
Research Associate II
CovX
Ph. (858)964-2050
Fax (858)964-2090
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Message: 24
Date: Fri, 8 Dec 2006 09:54:09 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: [Histonet] employment
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002d01c71ae1$18f9b410$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain; charset="us-ascii"
Hi All,
A histotech here in the Houston area asked me to place a post on her behalf.
She is looking for employment, temporary or permanent. The Houston area
would be great, but she will consider other areas. She does not want to
work the night shift, however. If you have a position available, please
email me off-line and I will forward the messages.
Thanks,
Bonnie Whitaker
Lab Manager
Brown & Associates Medical Laboratories
8076 El Rio
Houston, Texas 77054
713-741-6677
------------------------------
Message: 25
Date: Fri, 8 Dec 2006 16:01:50 -0000
From: "Colin Nixon" <csn1x <@t> udcf.gla.ac.uk>
Subject: [Histonet] CD 79 antibody
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002601c71ae2$2bd18170$d7e9d182 <@t> vet.gla.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue?
Colin
------------------------------
Message: 26
Date: Fri, 8 Dec 2006 08:08:25 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] CD 79 antibody
To: Colin Nixon <csn1x <@t> udcf.gla.ac.uk>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <412456.26375.qm <@t> web61221.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I used CD79a from Novocastra (1:25 dil pH6 HIER), tonsil control.
René J.
Colin Nixon <csn1x <@t> udcf.gla.ac.uk> wrote:
Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue?
Colin
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Message: 27
Date: Fri, 8 Dec 2006 10:11:41 -0600
From: "Breckenridge, Richard A" <Richard.Breckenridge <@t> uth.tmc.edu>
Subject: [Histonet] digital image analysis systems...
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F7D64ED7B9F94141B07414ED2E2E796E0330AFCF <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
Just looking for feedback on image analysis systems that are out there.
I plan on demo-ing some and any info is welcome. Thanks!
Rick
Richard A. Breckenridge, HT(ASCP)
Technical Director/ Chief Histology Technician
University of Texas- Houston Medical School
Histology/ IHC Laboratory
Office 713-500-6792 IHC Lab 713-500-5096
Histology Lab 713-500-5363 FAX 713-500-0733
Email - Richard.Breckenridge <@t> uth.tmc.edu
------------------------------
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