[Histonet] mouse antibodies

[Gayle Callis]

And the CD4 and CD8 will not work on formalin fixed paraffin sections even 
after retrievals or digestion. These need to be done on frozen sections 
from fresh snap frozen tissues.  There may other CD markers mentioned that 
do not work on FFPE either.  Sorry to be a wet blanket

At 06:46 AM 12/11/2006, you wrote:
>Linda,
>
>Serotec and BD Pharmingen have a lot of anti-mouse antibodies.  These 2 
>vendors would be a good place to start.
>
>Good luck,
>Kim
>
>Kim Merriam, MA, HT(ASCP)
>Amgen
>Cambridge, MA
>
>
>
>----- Original Message ----
>From: LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com>
>To: histonet <@t> lists.utsouthwestern.edu
>Cc: ANA GOMEZ <ANA.GOMEZ <@t> childrens.com>
>Sent: Friday, December 8, 2006 3:57:57 PM
>Subject: [Histonet] mouse antibodies
>
>
>Dear Histonetters:
>My colleagues are are looking for a good supplier of anti-mouse
>antibodies for immunohistochemistry.  They already have anti-CD3 and
>anti-CD19, but need anti-CD4, anti-CD8, anti-macrophage/monocyte,
>anti-plasma cells, and anti-eosinophils.  These are for studies in
>formalin-fixed paraffin sections of mouse tissues.
>Any suggestions?  Thanks so much,
>Linda M
>Histonet administrator
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)