[Histonet] GFP and B-Gal positive control slides

Koelling, Ray koellinr <@t> amgen.com
Fri Dec 8 11:43:30 CST 2006


The GFP antibodies we use are quirky so I don't want to make a
recommendation.  At Jax where you can get the Tie-2/lacZ transgenic mice,
you can also get GFP expressing mice for controls.  I'm not recommending,
just a starting point for your search.  Look under research models on their
web site.

For the Tie-2/lac Z mice.  Fix in formalin and process to paraffin as usual.
We use an antibody from Cortex Biochem (San Leandro, CA) that is IgG
fraction on rabbit polyclonal to beta-galactosidase.  Around 2-3 ug/ml,
after a 5 min proteinase K digestion.  We use an amplification kit of the
third step (TSA for 5 minutes).  DAB, etc.  The signal is outstanding.


Raymond Koelling
Research Scientist
Amgen Seattle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Sent: Thursday, December 07, 2006 1:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GFP and B-Gal positive control slides

Does anyone know where I can buy, or can suggest an easy way to make  
positive control slides for GFP and B-Gal.

We have use AAV contstructs with either GFP or LAC-Z and injected  
into mice.  We have sectioned the tissue and would like to look for  
positive GFP or B-Gal.  It would be nice to have a positive control  
slide.  We plan on visualizing both with and without antibody  
amplification.  I would appreciate any suggestions.

Thank You.
Rebecca Nishi
UC Irvine
Christopher Reeve Foundation SCI Core/Anderson Lab
1226 GNRF
Irvine, CA 92697-4540
rnishi <@t> uci.edu
Phone/Fax: 949-824-9728

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