[Histonet] neurospheres

[Tarango, Mark]

When I take the chamber/divider thing off the slide, I make sure to
remove the media first (and very carefully so the cells don't come off
the slide).  Then I make sure to let it air dry for plenty of time
before I do anything to it.  If she is trying to use chamber slides on a
cell culture, I hope they are adherent cells.  If they are the kind of
cells that grow in suspension (like hematopoietic cells), then you won't
many cells adhere.  I don't know if neurospheres will adhere...

 

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska
Gentry
Sent: Thursday, December 07, 2006 8:19 AM
To: Histonet
Subject: [Histonet] neurospheres

Hello, does anyone have a protocol for fixation & staining neurospheres 
from tissue culture either on chamber slides or cryosections? If so will

you be so kind as to share it with me ASAP? One of our researchers here 
has attempted using the chamber slides but, she said the cells all 
washed off. Technique / protocol suggestions will be much appreciated. 
Thanks, Atoska

-- 
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu


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"EMF <nvcancer.org>" made the following annotations.
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