[Histonet] GFP and B-Gal positive control slides

Koelling, Ray koellinr <@t> amgen.com
Thu Dec 7 16:05:03 CST 2006

For GFP, that can be a can of worms.  For B-Gal this is what we do.  From
mouse vendor, get a Tie-2/lacZ transgenic mouse strain.  Are readily
available commercially.  Tie-2 is expressed in endothelium and the lacZ
reporter gene is co-expressed there.  Take any tissue (kid/liver/whatever).
B-Gal antibodies show beautifully stained endothelium. 

Raymond Koelling
Research Scientist
Amgen Seattle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Sent: Thursday, December 07, 2006 1:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GFP and B-Gal positive control slides

Does anyone know where I can buy, or can suggest an easy way to make  
positive control slides for GFP and B-Gal.

We have use AAV contstructs with either GFP or LAC-Z and injected  
into mice.  We have sectioned the tissue and would like to look for  
positive GFP or B-Gal.  It would be nice to have a positive control  
slide.  We plan on visualizing both with and without antibody  
amplification.  I would appreciate any suggestions.

Thank You.
Rebecca Nishi
UC Irvine
Christopher Reeve Foundation SCI Core/Anderson Lab
1226 GNRF
Irvine, CA 92697-4540
rnishi <@t> uci.edu
Phone/Fax: 949-824-9728

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