[Histonet] GFP and B-Gal positive control slides
koellinr <@t> amgen.com
Thu Dec 7 16:05:03 CST 2006
For GFP, that can be a can of worms. For B-Gal this is what we do. From
mouse vendor, get a Tie-2/lacZ transgenic mouse strain. Are readily
available commercially. Tie-2 is expressed in endothelium and the lacZ
reporter gene is co-expressed there. Take any tissue (kid/liver/whatever).
B-Gal antibodies show beautifully stained endothelium.
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Sent: Thursday, December 07, 2006 1:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GFP and B-Gal positive control slides
Does anyone know where I can buy, or can suggest an easy way to make
positive control slides for GFP and B-Gal.
We have use AAV contstructs with either GFP or LAC-Z and injected
into mice. We have sectioned the tissue and would like to look for
positive GFP or B-Gal. It would be nice to have a positive control
slide. We plan on visualizing both with and without antibody
amplification. I would appreciate any suggestions.
Christopher Reeve Foundation SCI Core/Anderson Lab
Irvine, CA 92697-4540
rnishi <@t> uci.edu
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