[Histonet] RE: decreased staining on controls

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Wed Dec 6 10:41:45 CST 2006

I have noticed the same but, as I always cut my control sections fresh with
the test (they go on the same slide), I am sure that it's the control blocks
that deteriorate with age. Coincidentally, I did a test run today on all my
CD 15 blocks in store to compare with the positive control check slides I
did on the same blocks back in Sept '05 (I keep all my pos control check
slides for future reference). This is because the other day I trialled some
CD 15 from a different supplier (but same clone) and found that the current
CD 15 pos block seemed to stain rather weaker with both my routine and new
CD 15 antisera compared to the results I got for that same block in Sept
The results showed a reduction in the staining intensity.
Happily (but not for the patient) we have just had a Hodgkin's case in which
was rip-roaringly CD 15 positive so I can replenish my store. 
I have also noticed the same with CD 30 in the past. 

Jacqui malam

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