[Histonet] Histonet] Tube cross sections in Frozens

Stephen Peters M.D. petepath <@t> yahoo.com
Mon Dec 4 12:31:57 CST 2006

Dear Renee,
  This can be accomplished easily using frozen block cryoenmbedding. This is a two step 
  embedding process. First the tissue is frozen in a block of embedding medium. This now firm easy to cut frozen block is then sectioned on edge. The resulting slices are then embedded on edge  either face down in a well  ( mold) or just by lying it face up on a chuck. You can do the first freeze between any two pieces of freezing temp metal. This process is detailed at: 

Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 

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