[Histonet] F4/80 quick question

[Gayle Callis]

Yes, and it works very well.  It is in our panel of murine antibodies.  Our 
frozen sections are air dried overnight then fixed in acetone 75%/ absolute 
ethanol 25% 5 min at RT,  then directly into buffer after fixation without 
any further air drying.

We did a dilution panel for our laboratory conditions,  and used a goat 
antiRat F(ab')2 secondary, Strepavidin-HRP/AEC with all the appropriate 
blocking step.

  We use this antibody for both immunohistochemistry and immunofluorescence 
staining.

At 09:47 AM 12/4/2006, you wrote:
>Does F4/80 from Serotec work on frozen sections of mouse tissue?
>--

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)