[Histonet] Decreased Staining on Controls

Annette Hall Annette_hall <@t> pa-ucl.com
Mon Dec 4 12:20:55 CST 2006


Hi,

We are having problems with are CD15 and CD30 antibodies using the
DakoCytomation Artisan. The controls seem lighter than when we first started
with this instrument 4 years ago. Our instruction has been to use "fresh"
controls. We cut controls and store them at 4 deg C for a few months. In
your experience, what do you consider fresh? How long do you keep cut slides
controls before you toss them?

Thanks,
Annette J Hall, MT
Micro/Cyto/Histo Supervisor
United Clinical Labs
205 Bluff Street
Dubuque, IA 52001
Phone: 563.556.2010 x131



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