[Histonet] Non Formalin fixatives

pmarcum <@t> vet.upenn.edu pmarcum <@t> vet.upenn.edu
Mon Dec 4 09:49:35 CST 2006

Gayle is right it can reduce your disposal cost and will pay for itself in a
short period of time.  Also with formalin subs you have to be sure none of your
antibodies are linked to an FDA protocol that requires formalin as the fixative
for a specific drug to be given based on the test results.  You would need to
re-titer or check every antibody against your formalin results to be sure they
will not need adjustments.  I am hoping to begin recylcling next year dispite
the fact we get our alcohols through the university for a very good price.  The
cost of xylene and xylene substiutes is still high.

Pam Marcum

Quoting Gayle Callis <gcallis <@t> montana.edu>:

> Why not consider purchasing recyclers for solvents and for formalin. People 
> or labs using these like them and have reduced both the cost of collection, 
> packaging and shipping wastes considerably. You still need to use absolute 
> alcohols since recycling only produces alcohols to 95%.  Propar and 
> Clearite 3 can be recyled also.   Check out the archives on recycling, 
> there has been discussion on this many time.
> At 02:31 PM 12/3/2006, you wrote:
> >Hi all,
> >I am trying to reduce the volume of formalin and solvent waste in my Lab. 
> >Has anyone had experience and comments on non formalin fixatives? Also, I 
> >have been able to use Aqua DePar from Biocare to deparaffinize my sections 
> >for H&E, special stains and immunohistochemistry with great results but 
> >would also like to try an H&E without alcohol differentiation and 
> >dehydration. Does anyone know of a H&E procedure that does not use alcohol 
> >after eosin?
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> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
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