[Histonet] positioning tissues in OCT

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Fri Dec 1 13:52:30 CST 2006

Double embed them.  Embed them flat - when that block hardens,  trim and 
turn it 90 degrees to where you can re-embed it so you get the desired 

"Till, Renee" <TillRenee <@t> uams.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/01/2006 01:25 PM

histonet <@t> lists.utsouthwestern.edu

[Histonet] positioning tissues in OCT

For our paraffin blocks we embed rat gi on end so when sectioned you get
a little circle of tissue. Any suggestions on how to do this for
cryosections?  The fresh tissues have been too soft and flexible to stay
standing up in the OCT until it freezes. Thanks.


Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72202

Office (501)364-2785

Fax (501)364-3161


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