[Histonet] Frozen tissue storage

Hofecker, Jennifer L jennifer.l.hofecker <@t> Vanderbilt.Edu
Thu Aug 31 13:49:49 CDT 2006

Hi Matthew, I am an alumni of the ziplock bags full of "goodies" in the -80 freezer, too!
We implemented a new system last year.  We got 30 ml specimen cups from Surgipath (may get from another vendor too).  They are actually the same vials that come prefilled or prelabeled.  We were able to get them with no labels and no formalin.  They come in a box of 50 that is the exact same size as the depth of our (upright) freezer shelf.  We wrap the OCT in foil and then label one of the specimen cups with the number ( on the lid too) Then we label the outside of the box.  We can open the door, grab a specimen and be out of the freezer before you know it.  If you have a surgipath rep, they can probably get you a sample box (that's how we fell in love...)
BEFORE THE FLAMING:  I know there are oodles of vendors of specimen containers.  This box size is perfect and there is a cardboard insert inside that makes it super easy to take one out and put it back in the same spot.
Good Luck and Have a great Rest of the week!
Jennifer Hofecker, HT (ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
(615) 343-0083
(615) 343-7089 fax

Mathew DeGutes <m-degutes <@t> northwestern.edu> wrote:
  I have a couple questions. In the lab I work in we have started to
accumulate a number of experiments worth of tissue blocks that we would
like to keep for reference at later dates. Generally tissue survives
better while still embedded in a block than when in slide form, so I was
curious as to what sorts of methods others here employ to keep order and
good preservation of blocks in -80s? Up until now we have been just
keeping individual sets of blocks in Ziplock bags, but it has gotten to
be unwieldy. I am looking for any suggestions of a good way to keep the
blocks well preserved and easily recovered in -80s.
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