[Histonet] Immunohistochem help!

[Brown, Graham W]

Dear All-
 
I'm a student with a little bit of experience in immunohistochemistry. I am familiar with using paraffin sections and fresh frozen sections, however i've never stained any tissue that was fixed prior to freezing. I was wondering if anyone has a general protocol for staining fixed (with formalin) frozen tissue. Is it better to use fresh tissue for frozens rather than using fixed frozen tissues? Thanks in advance!
 
Graham Brown
 
Texas Tech University Health Sciences Center