[Histonet] tissues falling off
Melissa Mazan
melissa.mazan <@t> tufts.edu
Sun Aug 27 20:39:12 CDT 2006
Tanni - I had the same problem - my citrate buffer was too alkaline - pH
of 6 made everything work fine. Melissa
histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Looking for a new vendor for Amyloid P Antibody (Nava, Josefa)
> 2. Disposal of surgical specimens (Linda Stirpe)
> 3. eNOS evaluation (AGrobe2555 <@t> aol.com)
> 4. Re: Disposal of surgical specimens (Rene J Buesa)
> 5. diener pay (Spoon, Victoria)
> 6. RE: Histonet Digest, Vol 33, Issue 28 (Margaryan, Naira)
> 7. Cost of consumables (tncperfect2 <@t> comcast.net)
> 8. Rat Histology (Hector Mobine)
> 9. Hydrogen peroxide sterilization (AGrobe2555 <@t> aol.com)
> 10. Histo Position (Barbara Murray)
> 11. Re: diener pay (Godsgalnow <@t> aol.com)
> 12. Re: Cost of consumables (Godsgalnow <@t> aol.com)
> 13. Re: Looking for a new vendor for Amyloid P Antibody
> (Jennifer MacDonald)
> 14. RE: Who cleans your mortuary? (Rosalba)
> 15. unsubscribe (Lynda King)
> 16. F4/80 (Helen Beard)
> 17. p-0 fixation and embedding-correction (Vicki Hakim)
> 18. RE: Disposal of surgical specimens (Molinari, Betsy)
> 19. IHC - sections falling off! (Ahmed, T (Tanni))
> 20. Re: Disposal of surgical specimens (Janci Wellborn)
> 21. RE: IHC - sections falling off! (Dawson, Glen)
> 22. Refurbished instruments with CMD (Coleman Manufacturing)
> 23. RE: Patient slide send-out (Richard Cartun)
> 24. RE: Disposal of surgical specimens (Horn, Hazel V)
> 25. Re: F4/80 (Gayle Callis)
> 26. RE: Refurbished instruments with CMD (Jes Strong)
> 27. Re: Cost of consumables (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 23 Aug 2006 12:50:30 -0500
> From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
> Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <2C515C1049EAF5459EFD8C9B929078A401DA13A6 <@t> phdex03.txhealth.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello,
>
> Can someone tell me where I can order Amyloid P antibody. Dako and
> Novocastra have discontinued it. I will appreciate any information you
> can give me. I will be running the Amyloid P on a Ventana machine and
> VBS Bond Max. Thank you.
>
> Josie
>
>
> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system.
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> ------------------------------
>
> Message: 2
> Date: Wed, 23 Aug 2006 10:54:51 -0700 (PDT)
> From: Linda Stirpe <lpstirpe <@t> yahoo.com>
> Subject: [Histonet] Disposal of surgical specimens
> To: HistoNet Server <histonet <@t> pathology.swmed.edu>
> Message-ID: <20060823175451.94510.qmail <@t> web54614.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I know this subject has been previously discussed but
> now it has become an issue at our facility. We are a
> small hospital processing about 5,000 surgicals/year.
> Currently, we dispose of the specimens with fixative
> by placing them in biohazard containers and then
> hauled off site. The safety committee now has concerns
> that the specimen is a biohazard and the formain is a
> hazardous waste and should be separated for disposal
> purposes. We are interested in how other hospitals
> handle this. Any suggestions would be greatly
> appreciated. Thank you!
>
> Linda Stirpe, HT(ASCP)
> Corning Hospital
> Corning, NY
>
>
> __________________________________________________
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> ------------------------------
>
> Message: 3
> Date: Wed, 23 Aug 2006 14:49:08 EDT
> From: AGrobe2555 <@t> aol.com
> Subject: [Histonet] eNOS evaluation
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <57c.3e0afac.321dfd24 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Tahseen,
> I have worked with eNOS in the past. Can you give me a bit more detail of
> what you are looking for? Cellular localization, levels, NO production,
> phosphorylation state, etc....?
> Albert
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 23 Aug 2006 11:54:26 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Disposal of surgical specimens
> To: Linda Stirpe <lpstirpe <@t> yahoo.com>, HistoNet Server
> <histonet <@t> pathology.swmed.edu>
> Message-ID: <20060823185426.21282.qmail <@t> web61218.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Linda:
> We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab.
> Hope this will help you!
> René J.
>
> Linda Stirpe <lpstirpe <@t> yahoo.com> wrote:
> I know this subject has been previously discussed but
> now it has become an issue at our facility. We are a
> small hospital processing about 5,000 surgicals/year.
> Currently, we dispose of the specimens with fixative
> by placing them in biohazard containers and then
> hauled off site. The safety committee now has concerns
> that the specimen is a biohazard and the formain is a
> hazardous waste and should be separated for disposal
> purposes. We are interested in how other hospitals
> handle this. Any suggestions would be greatly
> appreciated. Thank you!
>
> Linda Stirpe, HT(ASCP)
> Corning Hospital
> Corning, NY
>
>
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> ------------------------------
>
> Message: 5
> Date: Wed, 23 Aug 2006 15:22:06 -0400
> From: "Spoon, Victoria" <victoria.spoon <@t> bassett.org>
> Subject: [Histonet] diener pay
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <415700FC732DE14491A3E39367834F7715AD6E <@t> ex3.bassett.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> What is the going rate of pay for dieners?
>
> Thanks in advance.
>
>
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>
> ------------------------------
>
> Message: 6
> Date: Wed, 23 Aug 2006 16:38:08 -0500
> From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
> Subject: [Histonet] RE: Histonet Digest, Vol 33, Issue 28
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <63B8B599DE283148B92E83C78B32C15D02E0DFEE <@t> cmhexbe2.childrensmemorial.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Me too, please :-) And, please, not only histology, but cancer pathology
> as well!!!
>
>
>
> Thanks,
>
> Naira
>
>
>
> Naira V. Margaryan, D.V.M., Ph.D.
>
> Research Associate III
>
> Children's Memorial Research Center
>
> 2300 Children's Plaza, Box 222
>
> Chicago, IL 60614-3394
>
> Tel: 773-755-6570/ext-8
>
> Fax: 773-755-6594
>
> nmargaryan <@t> childrensmemorial.org
>
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>
> For Express Mail:
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>
> 2430 N. Halsted Street
>
> Chicago, IL 60614-4314
>
>
>
> ------------------------------
>
>
>
> Message: 10
>
> Date: Tue, 22 Aug 2006 17:53:49 -0800
>
> From: "Barbara Murray" <bamur <@t> alaska.net>
>
> Subject: [Histonet] On-line Histology Schools
>
> To: <histonet <@t> lists.utsouthwestern.edu>
>
> Message-ID: <EAEKKDHFCFKJHMBKDOCCGENACMAA.bamur <@t> alaska.net>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
>
>
>
>
> Greetings from Anchorage, Alaska
>
> Would someone please send me a list of on-line Histology Schools.
>
> Thanks in advance and have a great day!
>
>
>
> Barbara A. Murray, HT. (ASCP)
>
> The Alaska Native Medical Center
>
> Anchorage, Alaska
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 23 Aug 2006 22:09:24 +0000
> From: tncperfect2 <@t> comcast.net
> Subject: [Histonet] Cost of consumables
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <082320062209.25134.44ECD214000AC3560000622E2213575333CD9B0C0A009D0A9F0C029B <@t> comcast.net>
>
> Content-Type: text/plain
>
> Hi Histonetters.
> I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain.
> If anyone can help me it would be very much appreciated.
> Thanks,
> Carolyn
>
> ------------------------------
>
> Message: 8
> Date: Wed, 23 Aug 2006 18:16:46 -0400
> From: "Hector Mobine" <mobine <@t> MIT.EDU>
> Subject: [Histonet] Rat Histology
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <200608232216.k7NMGkix025572 <@t> outgoing.mit.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I am in the process of designing histological experiments of rat and mice
> hearts and would be interested in a good description of rat heart excision
> and anatomy. I have yet been unable to find one in medical textbooks,
> reviews or protocols. Does anyone know of any good references, pictures,
> books or protocols? Any help would be appreciated.
>
>
>
> Thanks,
>
> Hector
>
>
>
>
>
> Graduate Student
>
> Edelman Lab
>
> Biological Engineering Division
>
> Massachusetts Institute of Technology
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 23 Aug 2006 18:27:22 EDT
> From: AGrobe2555 <@t> aol.com
> Subject: [Histonet] Hydrogen peroxide sterilization
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <414.8c46d5a.321e304a <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Does anyone know of a company which will do H2O2 gas-phase sterilization on
> a contract basis? Please feel free to contact me directly off-list.
> Thanks,
> Albert
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 23 Aug 2006 15:29:15 -0800
> From: "Barbara Murray" <bamur <@t> alaska.net>
> Subject: [Histonet] Histo Position
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <EAEKKDHFCFKJHMBKDOCCOENHCMAA.bamur <@t> alaska.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
> Greetings from Anchorage, Alaska.
> We currently have two openings in Histology @ The Alaska Native Medical
> Center. Contact David Morrison, Laboratory Manager @ 907-729-1228 for
> details and $$$$$$!
> Have a great day!
>
> Barbara A. Murray, HT. (ASCP)
> The Alaska Native Medical Center
> Anchorage, Alaska 99508
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 23 Aug 2006 20:56:22 EDT
> From: Godsgalnow <@t> aol.com
> Subject: Re: [Histonet] diener pay
> To: victoria.spoon <@t> bassett.org, histonet <@t> pathology.swmed.edu
> Message-ID: <516.60e833f.321e5336 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> I have always paid per case.....$250 - $300 per case. It doesn't matter if
> it is a chest only or a full body, the pay is the same.
> Roxanne
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 23 Aug 2006 21:01:19 EDT
> From: Godsgalnow <@t> aol.com
> Subject: Re: [Histonet] Cost of consumables
> To: tncperfect2 <@t> comcast.net, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <37d.9d06a47.321e545f <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> This is all so subjective. It depends on where you are located, how much
> you pay for shipping, if at all. If you have a GPO or multiple vendors that
> you use. Progressive or regressive staining? Gills, Harris, Clarifier or no?
> Bluing or no? Plus slides, regular slides? Do you cut extras? Do you have
> to pay for DI water? Do you have a specific brand of products you use?
>
>
> Roxanne Soto
> Lab Manager
> Physicians RightPath
> Tampa, Florida
> 813-549-1050
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 23 Aug 2006 20:32:12 -0700
> From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Subject: Re: [Histonet] Looking for a new vendor for Amyloid P
> Antibody
> To: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
> Cc: histonet <@t> pathology.swmed.edu
> Message-ID:
> <OFBAC3711C.88922F3A-ON882571D4.00136DC9-882571D4.00136DCC <@t> mtsac.edu>
> Content-Type: text/plain; charset="UTF-8"
>
>
> I know that BioCare has it. You can also check Cell Marque.
>
>
>
>
> -----hist To: <histonet <@t> pathology.swme From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
> Sen Date: 08/23/2006 10:50AM Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody
> Hello,
> Can someone tell me where I can order Amyloid and
> Novocastra have discontinued &nb information you
> can give me. I will be runn and
> VBS Bond Max. &nb Josie
> The information contained in this mes intended only for the use of the individual or addressed, and may contain information that is PRIVIL CONFIDENTIAL, and exempt from disclosure under applicable law. you are not the intended recipient, you are prohibited from copying,
> dis immedi your system.
> _______________________ 5F _______________________
> Hi Histonet <@t> lists.utsouthwestern.edu
> [1]htt
> References
>
> 1. file://localhost/tmp/3D"http
>
> ------------------------------
>
> Message: 14
> Date: Thu, 24 Aug 2006 15:39:39 +1000
> From: Rosalba <zumbor <@t> email.cs.nsw.gov.au>
> Subject: RE: [Histonet] Who cleans your mortuary?
> To: 'Mike Kirby' <mike.kirby <@t> nhls.ac.za>, "Histonet (E-mail)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <01C6C793.83411BC0.zumbor <@t> email.cs.nsw.gov.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi All,
> The mortuary technicians who assist with the post-mortems clean the
> mortuary after they have finished their cases. Each cleans their own work
> area and there is a roster for cleaning the other general areas such as the
> fridge etc.
> Rosalba Zumbo
> Supervisor Histology Dept
> Department of Forensic Medicine
> 42-50 Parramatta Rd
> Glebe NSW 2037
> Australia
> PH: 61 2 85847842
> FAX: 61 2 95664573
>
>
>
> -----Original Message-----
> From: Mike Kirby [SMTP:mike.kirby <@t> nhls.ac.za]
> Sent: Wednesday, 23 August 2006 7:45 PM
> To: Histonet (E-mail)
> Subject: [Histonet] Who cleans your mortuary?
>
>
> Hi Guys.
>
> Question! Who cleans your mortuary? Swabbing the floors and tables,
> cleaning the refrigeration area, sinks, windows, etc. Do you have
> permanent, in-house trained Staff, or do you contract out to a commercial
> company?
>
> M.Kirby
> Johannesburg
> South Africa.
>
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> ------------------------------
>
> Message: 15
> Date: Thu, 24 Aug 2006 16:15:45 +1000
> From: "Lynda King" <Lynda.King <@t> vision-bio.com>
> Subject: [Histonet] unsubscribe
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <5E06BFED29594F4C9C5EBE230DE320C6114B5DBC <@t> ewok.vsl.com.au>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
> Lynda King
> Customer and Sales Administrator
>
> Vision BioSystems Ltd
> 495 Blackburn Road
> Mt Waverley VIC 3149
> Australia
>
> telephone: +61 3 9211 7400
> facsimile: +61 3 9211 7401
>
> www.vision-bio.com <http://www.vision-bio.com>
>
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>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 24 Aug 2006 03:18:32 -0500
> From: "Helen Beard" <hbeard27 <@t> hotmail.com>
> Subject: [Histonet] F4/80
> To: mobine <@t> MIT.EDU, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY103-F156C8A6787585F3E760D93D2440 <@t> phx.gbl>
> Content-Type: text/plain; format="flowed"
>
>
> I would be interested in hearing from anyone who has used Sertec F4/80
> to do IHC for microglia in pfa fixed mouse brains. I have lovely
> staining in my kupfer cells (control) but only staining of what
> appears to be neurones in the brain. A protocol would be greatly
> appreciated.
>
> Thanks in advance
>
> Helen
>
>
> ------------------------------
>
> Message: 17
> Date: Thu, 24 Aug 2006 09:52:28 GMT
> From: Vicki Hakim <hakim <@t> bgu.ac.il>
> Subject: [Histonet] p-0 fixation and embedding-correction
> To: Gayle Callis <gcallis <@t> montana.edu>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <f496b5ded088.44ed76dc <@t> bgu.ac.il>
> Content-Type: text/plain; charset=utf-8
>
> sorry for the abbreviation, p-0 means p- zero (immediately after birth)
> thanks again
> vicki
> ----- Original Message -----
> From: Gayle Callis <gcallis <@t> montana.edu>
> Date: Wednesday, August 23, 2006 19:03
> Subject: Re: [Histonet] p-0 fixation and embedding
> To: Vicki Hakim <hakim <@t> bgu.ac.il>
>
>>Define what P-O mouse brains means, please.
>>
>>At 08:26 AM 8/23/2006, you wrote:
>>
>>>hi all
>>>we are interested in a protocol for fixation and embedding for
>>
>>P-0 mouse
>>
>>>brains. if u have a recommendation on 1) the preferable
>>
>>fixative (PFA or
>>
>>>formalin) and the specific times for fixation with each
>>
>>substance 2) time
>>
>>>in the melted paraffin 3) xylene or toluene
>>>thanks in advance
>>>vicki
>>>
>>>
>>> ‎
>>>_______________________________________________
>>>Histonet mailing list
>>>Histonet <@t> lists.utsouthwestern.edu
>>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>Gayle Callis
>>MT,HT,HTL(ASCP)
>>Research Histopathology Supervisor
>>Veterinary Molecular Biology
>>Montana State University - Bozeman
>>PO Box 173610
>>Bozeman MT 59717-3610
>>406 994-6367
>>406 994-4303 (FAX)
>>
>>
>>‎
>
>
>
> ------------------------------
>
> Message: 18
> Date: Thu, 24 Aug 2006 06:09:26 -0500
> From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
> Subject: RE: [Histonet] Disposal of surgical specimens
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C124B59C51D3D447878265D53492834E023BCE73 <@t> thimail.THI2.COM>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Linda,
> We do the same here. Our tissue is stored in "seal a meal" Heavy Duty Kapak bags until the investigator is ready to let it go. Then we cut a small corner off the bag, dump the formalin in a waste container, reseal the bag and put it into a biohazard bag for the hospital to dispose of.
>
> Betsy Molinari HT (ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 6770 Bertner Ave.
> Houston,TX 77030
> 832-355-6524
> 832-355-6812 (fax)
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Wednesday, August 23, 2006 1:54 PM
> To: Linda Stirpe; HistoNet Server
> Subject: Re: [Histonet] Disposal of surgical specimens
>
> Linda:
> We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab.
> Hope this will help you!
> René J.
>
> Linda Stirpe <lpstirpe <@t> yahoo.com> wrote:
> I know this subject has been previously discussed but
> now it has become an issue at our facility. We are a
> small hospital processing about 5,000 surgicals/year.
> Currently, we dispose of the specimens with fixative
> by placing them in biohazard containers and then
> hauled off site. The safety committee now has concerns
> that the specimen is a biohazard and the formain is a
> hazardous waste and should be separated for disposal
> purposes. We are interested in how other hospitals
> handle this. Any suggestions would be greatly
> appreciated. Thank you!
>
> Linda Stirpe, HT(ASCP)
> Corning Hospital
> Corning, NY
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
>
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Want to be your own boss? Learn how on Yahoo! Small Business.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 19
> Date: Thu, 24 Aug 2006 12:15:55 +0100
> From: "Ahmed, T \(Tanni\)" <Tanni.Ahmed <@t> intervet.com>
> Subject: [Histonet] IHC - sections falling off!
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <11DBF8AFF1DC0F4A9B51A23F8E5BDA33799067 <@t> mksn83.d50.intra>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear All,
>
> I am trying to save sections of canine mammary tissue from falling off
> in antigen retrieval solution in a microwave technique (have also tried
> half power microwaving). I am using normal superfrost slides and leaving
> them to incubate overnight. The sections are fine after dewaxing -
> therefore I believe the type of slide used whether it be superfrost or
> positive charged polysaline slides should not matter? My other option is
> maybe to use non heat-treatment methods of enzyme digestion such as
> trypsin. Is there anyone who has any ideas to prevent sections falling
> off during the microwave retrieval step?
>
> Any suggestions to improve are welcome.
>
> Thanks in advance,
>
> Tanni
>
> --------------------------------------
> This message, including attachments, is confidential and may be privileged.
> If you are not an intended recipient, please notify the sender then delete
> and destroy the original message and all copies. You should not copy, forward
> and/or disclose this message, in whole or in part, without permission of
> the sender.
> --------------------------------------
>
> ------------------------------
>
> Message: 20
> Date: Thu, 24 Aug 2006 08:18:03 -0400
> From: "Janci Wellborn" <wellborj <@t> mercyhealth.com>
> Subject: Re: [Histonet] Disposal of surgical specimens
> To: histonet <@t> pathology.swmed.edu, lpstirpe <@t> yahoo.com,
> rjbuesa <@t> yahoo.com
> Message-ID: <s4ed60c3.084 <@t> nodcmsngwia1.trinity-health.org>
> Content-Type: text/plain; charset=iso-8859-1
>
> Linda,
> We separate the formalin from the tissue. The formalin is then neutralized and solidified with product called ISOSORB. The tissue and solid formalin waste is then doubled bagged and disposed of as any other biohazard sample. Our limbs are incinerated by a local mortuary.
>
> Janci Wellborn, HTL, BS, BSeD
> Dunes Medical Lab
> Dakota Dunes, SD
>
>
>>>>"Rene J Buesa" <rjbuesa <@t> yahoo.com> 8/23/2006 1:54 PM >>>
>
> Linda:
> We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab.
> Hope this will help you!
> René J.
>
> Linda Stirpe <lpstirpe <@t> yahoo.com> wrote:
> I know this subject has been previously discussed but
> now it has become an issue at our facility. We are a
> small hospital processing about 5,000 surgicals/year.
> Currently, we dispose of the specimens with fixative
> by placing them in biohazard containers and then
> hauled off site. The safety committee now has concerns
> that the specimen is a biohazard and the formain is a
> hazardous waste and should be separated for disposal
> purposes. We are interested in how other hospitals
> handle this. Any suggestions would be greatly
> appreciated. Thank you!
>
> Linda Stirpe, HT(ASCP)
> Corning Hospital
> Corning, NY
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Want to be your own boss? Learn how on Yahoo! Small Business.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Thu, 24 Aug 2006 08:14:10 -0500
> From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
> Subject: RE: [Histonet] IHC - sections falling off!
> To: "Ahmed, T \(Tanni\)" <Tanni.Ahmed <@t> intervet.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B3D65550856D0146B900D401EE313D4B3F741C <@t> dynams.dynacaremilwaukee.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Tanni,
>
> Don't underestimate the power of a slide with extra adhesive properties. Try out Goldfrost Plus Slides or the Silanized slides from Dako.
>
> Also, try extending the time that you dry your slides before you start your procedure.
>
> Keep in mind that enzyme digestion can make tissue sections fall off as well.
>
> Good Luck,
>
> Glen Dawson BS, HT & QIHC (ASCP)
> IHC Manager
> Milwaukee, WI
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Ahmed, T
> (Tanni)
> Sent: Thursday, August 24, 2006 5:16 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC - sections falling off!
>
>
> Dear All,
>
> I am trying to save sections of canine mammary tissue from falling off
> in antigen retrieval solution in a microwave technique (have also tried
> half power microwaving). I am using normal superfrost slides and leaving
> them to incubate overnight. The sections are fine after dewaxing -
> therefore I believe the type of slide used whether it be superfrost or
> positive charged polysaline slides should not matter? My other option is
> maybe to use non heat-treatment methods of enzyme digestion such as
> trypsin. Is there anyone who has any ideas to prevent sections falling
> off during the microwave retrieval step?
>
> Any suggestions to improve are welcome.
>
> Thanks in advance,
>
> Tanni
>
> --------------------------------------
> This message, including attachments, is confidential and may be privileged.
> If you are not an intended recipient, please notify the sender then delete
> and destroy the original message and all copies. You should not copy, forward
> and/or disclose this message, in whole or in part, without permission of
> the sender.
> --------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 22
> Date: Thu, 24 Aug 2006 06:18:31 -0700 (PDT)
> From: Coleman Manufacturing <coleman_manufacturing <@t> yahoo.com>
> Subject: [Histonet] Refurbished instruments with CMD
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20060824131831.20959.qmail <@t> web37614.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability.
>
> Equipment of the following types:
> Cover Slippers
> Cryostats
> Microtomes
> Embedding centers
> Tissue Processors
> Stainers
> Laboratory Microwave Systems
> You can request more information on the available equipment by contacting our office at:
>
> Coleman Manufacturing & Design
> 2852 Old Airport Road
> Winnsboro, SC 29180
>
> 803.633.2124 office
> 803.635.9401 fax
> Coleman_Manufacturing <@t> yahoo.com
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Thu, 24 Aug 2006 09:25:00 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: RE: [Histonet] Patient slide send-out
> To: "Betsy Molinari" <BMolinari <@t> heart.thi.tmc.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <44ED706C020000770000187A <@t> gwmail.harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Today, patients are more educated regarding health care issues and no
> longer settle for what their physician tells them. Here in the East,
> many patients want their diagnoses confirmed by major cancer centers in
> New York and Boston. Also, they may be looking for alternative
> treatments.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
>>>>"Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> 08/23/06 6:37 AM
>>>>
>
> Just curious..why this increase in patients asking for a second
> opinion?
>
> Betsy Molinari HT (ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 6770 Bertner Ave.
> Houston,TX 77030
> 832-355-6524
> 832-355-6812 (fax)
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Zajic
> Kari
> Sent: Tuesday, August 22, 2006 2:05 PM
> To: Richard Cartun; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Patient slide send-out
>
> Richard, you are not alone! We have also seen an increase in patient
> and
> physician requests for "send-outs". I am not sure of charging, but I
> have made a "send-out" policy for our department that all requests be
> put in writing (scripts for physicians)and faxed,inform the
> patient/office that it will at least be 48 hours before it can be sent
> out, they must provide a shipping service account (FEDEX, UPS) or
> physically pick up the slides. We have also been being charged for
> these
> consults by the other facility so we also inform them that if the
> insurance cannot be charged, they are responsible for the bill. It
> seems
> to work but we do run into some problems now and again. If they cannot
> provide an overnight shipping service, we will USPS mail them
> certified
> but that seems to take too long for their liking (here it's around a
> week).
> Not having enough staff to handle the sendouts is always a problem,
> hence the 48 hours..helps slightly.
>
> Kari :)
>
> Kari Marie Zajic HTL,MLT
> Histology Supervisor
> Palms West Hospital
> Pathology Department
> 13001 State Road Eighty
> Loxahatchee, Florida 33470
> phone: (561)798-6036
> telefax: (561)753-4298
> voicemail: (561)753-4299
> pager: (561)610-4949
> email: Kari.Zajic <@t> HCAHealthcare.com
>
> This email and any files transmitted with it may contain PRIVILEGED or
> CONFIDENTIAL
> information and may be read or used only by the intended recipient. If
> you are not the intended recipient of the email or any of its
> attachment, please be advised that you have received this email in
> error
> and that any use, dissemination, distribution, forwarding, printing,
> or
> copying of this email or any attached files is strictly prohibited. If
> you have received this email in error, please immediately purge it and
> all attachments and notify the sender by reply email or contact the
> sender at the number listed.
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
> Cartun
> Sent: Tuesday, August 22, 2006 2:49 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Patient slide send-out
>
>
> Our Anatomic Pathology Office is overwhelmed with requests from
> patients
> asking for their pathology slides to be sent to another medical
> institution (for second opinion, additional surgery or therapy, etc.).
>
> Is it legal to charge patients for this service? We don't have the
> personnel to handle these requests in a timely fashion and we can no
> longer afford to "eat" the shipping costs. Thank you.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 24
> Date: Thu, 24 Aug 2006 09:02:11 -0500
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> Subject: RE: [Histonet] Disposal of surgical specimens
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Linda Stirpe"
> <lpstirpe <@t> yahoo.com>, "HistoNet Server" <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D53 <@t> EMAIL.archildrens.org>
> Content-Type: text/plain; charset=iso-8859-1
>
> We pour the formalin off of the specimens. The specimens are discarded and hauled off by the waste company. The spent formalin is also taken by another waste company for disposal. It's a pain.
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall Slot 820
> Little Rock, AR 72202
>
> phone 501.364.4240
> fax 501.364.3912
>
> visit us on the web at: www.archildrens.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Wednesday, August 23, 2006 1:54 PM
> To: Linda Stirpe; HistoNet Server
> Subject: Re: [Histonet] Disposal of surgical specimens
>
> Linda:
> We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab.
> Hope this will help you!
> René J.
>
> Linda Stirpe <lpstirpe <@t> yahoo.com> wrote:
> I know this subject has been previously discussed but
> now it has become an issue at our facility. We are a
> small hospital processing about 5,000 surgicals/year.
> Currently, we dispose of the specimens with fixative
> by placing them in biohazard containers and then
> hauled off site. The safety committee now has concerns
> that the specimen is a biohazard and the formain is a
> hazardous waste and should be separated for disposal
> purposes. We are interested in how other hospitals
> handle this. Any suggestions would be greatly
> appreciated. Thank you!
>
> Linda Stirpe, HT(ASCP)
> Corning Hospital
> Corning, NY
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam? Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Want to be your own boss? Learn how on Yahoo! Small Business.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------------------------------------------------------
> The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you.
> ==============================================================================
>
>
>
>
> ------------------------------
>
> Message: 25
> Date: Thu, 24 Aug 2006 09:09:13 -0600
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] F4/80
> To: "Helen Beard" <hbeard27 <@t> hotmail.com>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20060824090747.01b39ca0 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> This has been answered on Histonet several times, just go to Archives and
> type in F4/80. Barb Wright is one who has given good info on using this
> antibody from Serotec. There will be other excellent replies also.
>
> At 02:18 AM 8/24/2006, you wrote:
>
>
>> I would be interested in hearing from anyone who has used Sertec F4/80
>> to do IHC for microglia in pfa fixed mouse brains. I have lovely
>> staining in my kupfer cells (control) but only staining of what
>> appears to be neurones in the brain. A protocol would be greatly
>> appreciated.
>>
>> Thanks in advance
>>
>> Helen
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 26
> Date: Thu, 24 Aug 2006 11:01:40 -0500
> From: "Jes Strong" <jestrong <@t> comcast.net>
> Subject: RE: [Histonet] Refurbished instruments with CMD
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003001c6c796$96c2abc0$0200a8c0 <@t> Jes>
> Content-Type: text/plain; charset="us-ascii"
>
> This guy just doesn't quit! I would hope that all Histonetters would boycott
> supporting his company until he stops his blatant misuse of the Histonet for
> personal marketing purposes. There are many very good companies out there
> that respect the intent of the Histonet and can provide you with quality
> products at competitive prices.
>
> Jes Strong
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Coleman
> Manufacturing
> Sent: Thursday, August 24, 2006 8:19 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Refurbished instruments with CMD
>
> Tto provide every laboratory and hospital with the opportunity to enjoy the
> many benefits of our products, CMD is delighted to offer factory-refurbished
> instruments at significantly discounted prices. All of these instruments are
> backed by a full one-year parts and service warranty. Offer subject to
> availability.
>
> Equipment of the following types:
> Cover Slippers
> Cryostats
> Microtomes
> Embedding centers
> Tissue Processors
> Stainers
> Laboratory Microwave Systems
> You can request more information on the available equipment by contacting
> our office at:
>
> Coleman Manufacturing & Design
> 2852 Old Airport Road
> Winnsboro, SC 29180
>
> 803.633.2124 office
> 803.635.9401 fax
> Coleman_Manufacturing <@t> yahoo.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 27
> Date: Thu, 24 Aug 2006 09:05:32 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Cost of consumables
> To: tncperfect2 <@t> comcast.net, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20060824160532.39614.qmail <@t> web61212.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Carolyn:
> I just finished a paper on costs that I am sending you under separate cover.
> The cost are per unit, just multiply them by your needs.
> René J.
>
> tncperfect2 <@t> comcast.net wrote:
> Hi Histonetters.
> I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain.
> If anyone can help me it would be very much appreciated.
> Thanks,
> Carolyn
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
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> Get on board. You're invited to try the new Yahoo! Mail Beta.
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 33, Issue 30
> ****************************************
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor of Large Animal Medicine
Director of Sports Medicine
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA 01536
Tel: 508-839-5395
Fax: 508-839-7922
Email: melissa.mazan <@t> tufts.edu
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