[Histonet] TMA Book

Charles Scouten cwscouten <@t> myneurolab.com
Fri Aug 25 14:59:09 CDT 2006

 Instrumedics is now a part of the Vibratome company.  

We would be pleased to help any TMA lab with tape transfer.  There are
separate kits for frozen or paraffin sections, and many labs around the
world are finding tape transfer valuable for paraffin  or frozen TMA.

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thom
Sent: Friday, August 25, 2006 10:59 AM
To: Virginia.Achstetter <@t> afip.osd.mil; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] TMA Book

The Beecher I believe is the best instrument on the market.  If you go
to my website I did a review on the Chemicon TMArrrayer.  It has
problems that make the array construction process complicated and
longer.  If you can use the beecher I would recommend it.  I tested the
Chemicon last year at my lab so I have used it and tried to work out the
problems with the company.  But Chemicon was not corporative.  I have
articles on how to construct TMAs that are very helpful to now techs.
The first page on my website, at the bottom, tell were the articles are.
They are in the Journal of Histology.  If you have questions please feel
free to contact me any time.

The tape method is good for frozens but the tape destroys paraffin
They are compressed and most times unreadable.  Tech that use the tape
usually have a hard time cutting in the first place.  A good tech should
be able to cut without much problem.  I have a section on tape method
problems on my website:  http://arrayworkshop.com/Notes_2.html
If you need help cutting I can explain some techniques I use to achieve
nice sections with TMA blocks.

I have tried to work with the Tape company, Instrumedics, but they were
not very helpful and they couldn't solve the poor sectioning problems.

Good luck,

>From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
>To: "Thom Jensen" <tissuearray <@t> hotmail.com>
>Subject: RE: [Histonet] TMA Book
>Date: Fri, 25 Aug 2006 11:22:20 -0400
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>You are the best resource so far.   I believe the microarryer that I
>will be using is a chemicon.  My present department has the beecher
>manual arrayer but we never used it.   You have DVD's available.  Do
>have a book with techniques?  Also, you prefer to cut the block just
>like a paraffin block.   So many people use the tape method.  Why do
>they prefer tape?
>-----Original Message-----
>From: Thom Jensen [mailto:tissuearray <@t> hotmail.com]
>Sent: Friday, August 25, 2006 11:06 AM
>To: Achstetter, Virginia A.; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] TMA Book
>If you go to my website on creating tissuearrays I have several 
>resources on
>the subject.  Articles and videos.    www.arrayworkshop.com
>And I do answer question through email or phone.  I have given 
>workshops at two NSH meetings on tissue microarrays.
>Thom Jensen
> >From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
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> >Are there any good books out on how to prepare a tma block, trouble 
> >shooting, cutting techniques etc.
> >
> >Ginny Achstetter
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