[Histonet] CD123 and TMAs

[Thom Jensen]

Are you doing stains on a tma that was cut using the tape method?
Thom


>From: "Thomas Pier" <tp2 <@t> medicine.wisc.edu>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] CD123 and TMAs
>Date: Fri, 25 Aug 2006 11:09:56 -0500
>MIME-Version: 1.0
>Received: from swlx162.swmed.edu ([199.165.152.162]) by 
>bay0-mc6-f7.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Fri, 25 
>Aug 2006 09:10:42 -0700
>Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by 
>swlx162.swmed.edu with esmtp (Exim 4.34)id 1GGeGH-0002o8-RD; Fri, 25 Aug 
>2006 11:10:26 -0500
>Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by 
>swlx162.swmed.edu with esmtp (Exim 4.34) id 1GGeGE-0002ny-5Nfor 
>histonet <@t> lists.utsouthwestern.edu; Fri, 25 Aug 2006 11:10:23 -0500
>Received: from lists.medicine.wisc.edu 
>([128.104.208.5]helo=mailout.medicine.wisc.edu)by swlx167.swmed.edu with 
>esmtp (Exim 4.44) id 1GGeGA-0006tF-LFfor histonet <@t> lists.utsouthwestern.edu; 
>Fri, 25 Aug 2006 11:10:22 -0500
>Received: from gwmail.medicine.wisc.edu 
>(gwmail.medicine.wisc.edu[128.104.208.34])by mailout.medicine.wisc.edu 
>(8.13.1/8.13.1) with ESMTP idk7PGA82s006364for 
><histonet <@t> lists.utsouthwestern.edu>; Fri, 25 Aug 2006 11:10:13 -0500
>Received: from Medicine1-MTA by gwmail.medicine.wisc.eduwith 
>Novell_GroupWise; Fri, 25 Aug 2006 11:10:06 -0500
>X-Message-Info: LsUYwwHHNt26AsaAA0NCCboKDwfuXN5jlUkr4fQxcAc=
>X-Mailer: Novell GroupWise Internet Agent 7.0.1 
>X-UWMedicine-MailScanner-Information: Please contact the ISP for 
>moreinformation
>X-UWMedicine-MailScanner: Found to be clean
>X-UWMedicine-MailScanner-From: tp2 <@t> medicine.wisc.edu
>X-Scan-Signature: 2ab8d6fe94b74561233a4cdd2e311ef7
>X-BeenThere: histonet <@t> lists.utsouthwestern.edu
>X-Mailman-Version: 2.1.5
>Precedence: list
>List-Id: For the exchange of information pertaining to histotechnology 
>andrelated fields <histonet.lists.utsouthwestern.edu>
>List-Unsubscribe: 
><http://lists.utsouthwestern.edu/mailman/listinfo/histonet>, 
><mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=unsubscribe>
>List-Archive: <http://lists.utsouthwestern.edu/pipermail/histonet>
>List-Post: <mailto:histonet <@t> lists.utsouthwestern.edu>
>List-Help: <mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=help>
>List-Subscribe: 
><http://lists.utsouthwestern.edu/mailman/listinfo/histonet>,<mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=subscribe>
>Errors-To: histonet-bounces <@t> lists.utsouthwestern.edu
>X-Scan-Signature: 801ae9c9b293b3f34c4a36cf4e57e505
>X-SA-Exim-Connect-IP: 127.0.0.1
>X-SA-Exim-Mail-From: histonet-bounces <@t> lists.utsouthwestern.edu
>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu
>X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none 
>autolearn=no version=2.64
>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500)
>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu)
>Return-Path: histonet-bounces <@t> lists.utsouthwestern.edu
>X-OriginalArrivalTime: 25 Aug 2006 16:10:42.0247 (UTC) 
>FILETIME=[037BDD70:01C6C861]
>
>Hi everybody,
>I'm a little confused by my current situation.  I had worked up CD123
>(from BD) in a tonsil control and it looked great.  When I run the same
>condition on a lymphoma TMA the possitive control looks good and the TMA
>does not.  Has anybody out there had a similar problem or have any idea
>of what may be cuasing this?  Any help would be greatly appreciated.
>
>Tom Pier
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet