[Histonet] TMA Book

Thom Jensen tissuearray <@t> hotmail.com
Fri Aug 25 10:58:47 CDT 2006

The Beecher I believe is the best instrument on the market.  If you go to my 
website I did a review on the Chemicon TMArrrayer.  It has problems that 
make the array construction process complicated and longer.  If you can use 
the beecher I would recommend it.  I tested the Chemicon last year at my lab 
so I have used it and tried to work out the problems with the company.  But 
Chemicon was not corporative.  I have articles on how to construct TMAs that 
are very helpful to now techs.  The first page on my website, at the bottom, 
tell were the articles are.  They are in the Journal of Histology.  If you 
have questions please feel free to contact me any time.

The tape method is good for frozens but the tape destroys paraffin samples.  
They are compressed and most times unreadable.  Tech that use the tape 
usually have a hard time cutting in the first place.  A good tech should be 
able to cut without much problem.  I have a section on tape method problems 
on my website:  http://arrayworkshop.com/Notes_2.html
If you need help cutting I can explain some techniques I use to achieve nice 
sections with TMA blocks.

I have tried to work with the Tape company, Instrumedics, but they were not 
very helpful and they couldn't solve the poor sectioning problems.

Good luck,

>From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
>To: "Thom Jensen" <tissuearray <@t> hotmail.com>
>Subject: RE: [Histonet] TMA Book
>Date: Fri, 25 Aug 2006 11:22:20 -0400
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>You are the best resource so far.   I believe the microarryer that I
>will be using is a chemicon.  My present department has the beecher
>manual arrayer but we never used it.   You have DVD's available.  Do you
>have a book with techniques?  Also, you prefer to cut the block just
>like a paraffin block.   So many people use the tape method.  Why do
>they prefer tape?
>-----Original Message-----
>From: Thom Jensen [mailto:tissuearray <@t> hotmail.com]
>Sent: Friday, August 25, 2006 11:06 AM
>To: Achstetter, Virginia A.; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] TMA Book
>If you go to my website on creating tissuearrays I have several
>resources on
>the subject.  Articles and videos.    www.arrayworkshop.com
>And I do answer question through email or phone.  I have given workshops
>at two NSH meetings on tissue microarrays.
>Thom Jensen
> >From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
> >To: <histonet <@t> pathology.swmed.edu>
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> >Subject: [Histonet] TMA Book
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> >Are there any good books out on how to prepare a tma block, trouble
> >shooting, cutting techniques etc.
> >
> >Ginny Achstetter
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