AW: [Histonet] polyacids in trichrome stains

[Gudrun Lang]

 I know John Kiernan's book and the chapter about trichromes.  
But why has someone the idea that polyacids render the fibers coarse or
rough? This explanation is yet given to students. I am in doubt about it and
would like to disprove it with an experts' knowledge.

Gudrun

-----Ursprüngliche Nachricht-----
Von: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Gesendet: Montag, 21. August 2006 23:33
An: gu.lang <@t> gmx.at
Cc: Histonetliste (Histonetliste)
Betreff: Re: [Histonet] polyacids in trichrome stains

I seem to remember that Richard Horobin wrote a authoritative paper on this
subject more than a few years ago. Also, John Kiernan's textbook (and
website?) almost certainly offers an explanation.

Geoff

Gudrun Lang wrote:

>Hi!
>I've just read in a German textbook, that the polyacids 
>(phosphormolbydenacid, phosphortungstenacid) render the connective 
>tissue coarse to give the right staining in trichroms-methods. Is this 
>really a correct explanation? Or only an old theory (the book isn't 
>very old)? ;)
>
>Gudrun Lang
>
>
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